TY - JOUR
T1 - Alginate from the macroalgae Sargassum sinicola as a novel source for microbial immobilization material in wastewater treatment and plant growth promotion
AU - Yabur, Ricardo
AU - Bashan, Yoav
AU - Hernández-Carmona, Gustavo
N1 - Funding Information:
Acknowledgements We thank Juan-Pablo Hernandez, Manuel Moreno, and Patricia Vazquez from the Environmental Microbiology Group at CIBNOR for constant technical assistance and advice during the study. This study was partially supported by Secretaria del Medio Ambiente y Recursos Natu-rales of Mexico (SEMARNAT grant 2002-C01-0005) and the Bashan Foundation, USA. Author RY is a recipient of a graduate scholarship from the Consejo Nacional de Ciencia y Tecnolo-gia (CONACYT) and the Comisión de Operación y Fomento de Actividades Académicas del Instituto Politecnico Nacional (COFAA) of Mexico. Author GHC is a recipient of a fellowship from COFAA and EDI from the IPN in Mexico.
PY - 2007/2
Y1 - 2007/2
N2 - Alginate extracted from the macroalgae Sargassum sinicola was used as the raw material for co-immobilization of the microalgae Chlorella sorokiniana and growth-promoting bacterium Azospirillum brasilense for wastewater treatment and as an inoculant carrier of A. brasilense for plant growth promotion. The composition, structure, viscosity, color, and phenolic compound content of the alginate were analyzed and compared with commercially available alginate produced from the macroalgae Macrocystis pyrifera. From 1H NMR analysis of alginate, S. sinicola was found to have more guluronic acid (F G=0.64) than it had mannuronic acid (FM=0.38) and had a viscosity of 13.5 m Pa s compared to 50 m Pa s for M. pyrifera. The S. sinicola alginate had dark brown color, reducing light penetration, with more phenolic compounds than M. pyrifera alginate. Nonetheless, growth of C. sorokiniana and A. brasilense in S. sinicola alginate was not significantly different than the growth in M. pyrifera alginate beads. Nutrient removal from wastewater by the co-immobilized microorganisms was similar for both types of alginate beads, and so was the growth enhancement of tomato plants inoculated with microbeads containing A. brasilense. This study shows the potential use of S. sinicola alginate as a raw material for cell immobilization for wastewater treatment and plant growth promotion.
AB - Alginate extracted from the macroalgae Sargassum sinicola was used as the raw material for co-immobilization of the microalgae Chlorella sorokiniana and growth-promoting bacterium Azospirillum brasilense for wastewater treatment and as an inoculant carrier of A. brasilense for plant growth promotion. The composition, structure, viscosity, color, and phenolic compound content of the alginate were analyzed and compared with commercially available alginate produced from the macroalgae Macrocystis pyrifera. From 1H NMR analysis of alginate, S. sinicola was found to have more guluronic acid (F G=0.64) than it had mannuronic acid (FM=0.38) and had a viscosity of 13.5 m Pa s compared to 50 m Pa s for M. pyrifera. The S. sinicola alginate had dark brown color, reducing light penetration, with more phenolic compounds than M. pyrifera alginate. Nonetheless, growth of C. sorokiniana and A. brasilense in S. sinicola alginate was not significantly different than the growth in M. pyrifera alginate beads. Nutrient removal from wastewater by the co-immobilized microorganisms was similar for both types of alginate beads, and so was the growth enhancement of tomato plants inoculated with microbeads containing A. brasilense. This study shows the potential use of S. sinicola alginate as a raw material for cell immobilization for wastewater treatment and plant growth promotion.
KW - Algae
KW - Alginate
KW - Immobilization
KW - Macrocystis pyrifera
KW - Plant growth promoting bacteria
KW - Sargassum sinicola
KW - Wastewater treatment
UR - http://www.scopus.com/inward/record.url?scp=33847688921&partnerID=8YFLogxK
U2 - 10.1007/s10811-006-9109-8
DO - 10.1007/s10811-006-9109-8
M3 - Artículo
SN - 0921-8971
VL - 19
SP - 43
EP - 53
JO - Journal of Applied Phycology
JF - Journal of Applied Phycology
IS - 1
ER -