TY - JOUR
T1 - Aetiology of visceral leishmaniasis in Mexico
AU - Monroy-Ostria, Amalia
AU - Hernandez-Montes, Omar
AU - Barker, Douglas C.
N1 - Funding Information:
This work received the financial support of the Division de Estudios de Posgrado e Investigación IPN, Mexico and from Consejo Nacional de Ciencia y Tecnologia, Mexico (26503-N). A. Monroy-Ostria is sponsored by EDD and COFAA IPN, Mexico.
PY - 2000/3/25
Y1 - 2000/3/25
N2 - Two children with visceral leishmaniasis (VL), were studied by DNA analysis. DNA from liver biopsy samples from both patients, was amplified by PCR with broad primers specific for the Leishmania subgenus. DNA from the patient from Chiapas was also amplified with primers specific for the Leismania donovani complex and hybridised with a probe specific for L. donovani complex. The second patient, who is the first reported case of visceral leishmaniasis in the Mexican state of Tabasco, where localised cutaneous leishmaniasis and DCL predominate, had a co-infection with Toxoplasma gondii. The DNA from this patient was not amplified with primers specific for the L. donovani complex, did not hybridise with a probe specific for the L. donovani complex, but did hybridise with kDNA from a Mexican Leishmania mexicana strain used as a probe. We therefore, suggest that members of the L. donovani or L. mexicana complexes cause VL in Mexico. Copyright (C) 2000 Elsevier Science B.V.
AB - Two children with visceral leishmaniasis (VL), were studied by DNA analysis. DNA from liver biopsy samples from both patients, was amplified by PCR with broad primers specific for the Leishmania subgenus. DNA from the patient from Chiapas was also amplified with primers specific for the Leismania donovani complex and hybridised with a probe specific for L. donovani complex. The second patient, who is the first reported case of visceral leishmaniasis in the Mexican state of Tabasco, where localised cutaneous leishmaniasis and DCL predominate, had a co-infection with Toxoplasma gondii. The DNA from this patient was not amplified with primers specific for the L. donovani complex, did not hybridise with a probe specific for the L. donovani complex, but did hybridise with kDNA from a Mexican Leishmania mexicana strain used as a probe. We therefore, suggest that members of the L. donovani or L. mexicana complexes cause VL in Mexico. Copyright (C) 2000 Elsevier Science B.V.
KW - Aetiology
KW - L. (L.) chagasi
KW - L. (L.) mexicana
KW - Mexico
KW - Visceral leishmaniasis
UR - http://www.scopus.com/inward/record.url?scp=0034712429&partnerID=8YFLogxK
U2 - 10.1016/S0001-706X(99)00055-8
DO - 10.1016/S0001-706X(99)00055-8
M3 - Artículo
SN - 0001-706X
VL - 75
SP - 155
EP - 161
JO - Acta Tropica
JF - Acta Tropica
IS - 2
ER -