TY - JOUR
T1 - Aerobic biodegradation of a sulfonated phenylazonaphthol dye by a bacterial community immobilized in a multistage Packed-Bed BAC reactor
AU - Ruiz-Arias, Alfredo
AU - Juárez-Ramírez, Cleotilde
AU - De Los Cobos-Vasconcelos, Daniel
AU - Ruiz-Ordaz, Nora
AU - Salmerón-Alcocer, Angélica
AU - Ahuatzi-Chacón, Deifilia
AU - Galíndez-Mayer, Juvencio
N1 - Funding Information:
Acknowledgments We greatly appreciate the assistance of the personnel of the Spectroscopy and Microscopy Centrals, ENCB, IPN. This work was supported by a grant obtained from SIP, IPN.
PY - 2010/11
Y1 - 2010/11
N2 - A microbial community able to aerobically degrade the azo dye Acid Orange 7 was selected from riparian or lacustrine sediments collected at sites receiving textile wastewaters. Three bacterial strains, pertaining to the genera Pseudomonas, Arthrobacter, and Rhizobium, constitute the selected community. The biodegradation of AO7 was carried out in batch-suspended cell culture and in a continuously operated multistage packed-bed BAC reactor. The rapid decolorization observed in batch culture, joined to a delay of about 24 h in COD removal and cell growth, suggests that enzymes involved in biodegradation of the aromatic amines generated after AO7 azo-bond cleavage (1-amino-2-naphthol [1-A2N] and 4-aminobenzenesulfonic acid [4-ABS]), are inducible in this microbial consortium. After this presumptive induction period, the accumulated byproducts, measured through COD, were partially metabolized and transformed in cell mass. At all azo dye loading rates used, complete removal of AO7 and 1-A2N was obtained in the multistage packed-bed BAC reactor (PBR).; however, the overall COD (η COD ) and 4-ABS (η ABS ) removal efficiencies obtained in steady state continuous culture were about 90%. Considering the toxicity of 1-A2N, its complete removal has particular relevance. In the first stages of the packed-bed BAC reactor (Fig. 4a-c), major removal was observed. In the last stage, only a slight removal of COD and 4-ABS was obtained. Comparing to several reported studies, the continuously operated multistage packed-bed BAC reactor showed similar or superior results. In addition, the operation of large-packed-bed BAC reactors could be improved by using several shallow BAC bed stages, because the pressure drop caused by bed compaction of a support material constituted by small and fragile particles can be reduced.
AB - A microbial community able to aerobically degrade the azo dye Acid Orange 7 was selected from riparian or lacustrine sediments collected at sites receiving textile wastewaters. Three bacterial strains, pertaining to the genera Pseudomonas, Arthrobacter, and Rhizobium, constitute the selected community. The biodegradation of AO7 was carried out in batch-suspended cell culture and in a continuously operated multistage packed-bed BAC reactor. The rapid decolorization observed in batch culture, joined to a delay of about 24 h in COD removal and cell growth, suggests that enzymes involved in biodegradation of the aromatic amines generated after AO7 azo-bond cleavage (1-amino-2-naphthol [1-A2N] and 4-aminobenzenesulfonic acid [4-ABS]), are inducible in this microbial consortium. After this presumptive induction period, the accumulated byproducts, measured through COD, were partially metabolized and transformed in cell mass. At all azo dye loading rates used, complete removal of AO7 and 1-A2N was obtained in the multistage packed-bed BAC reactor (PBR).; however, the overall COD (η COD ) and 4-ABS (η ABS ) removal efficiencies obtained in steady state continuous culture were about 90%. Considering the toxicity of 1-A2N, its complete removal has particular relevance. In the first stages of the packed-bed BAC reactor (Fig. 4a-c), major removal was observed. In the last stage, only a slight removal of COD and 4-ABS was obtained. Comparing to several reported studies, the continuously operated multistage packed-bed BAC reactor showed similar or superior results. In addition, the operation of large-packed-bed BAC reactors could be improved by using several shallow BAC bed stages, because the pressure drop caused by bed compaction of a support material constituted by small and fragile particles can be reduced.
KW - 1-amino-2-naphthol
KW - 4-aminobenzenesulfonic acid
KW - Acid Orange 7
KW - Arthrobacter
KW - Biodegradation
KW - Biofilm
KW - Packed-bed reactor
KW - Rhizobium
KW - Sulfanilic acid
UR - http://www.scopus.com/inward/record.url?scp=78649654617&partnerID=8YFLogxK
U2 - 10.1007/s12010-010-8950-z
DO - 10.1007/s12010-010-8950-z
M3 - Artículo
C2 - 20376575
SN - 0273-2289
VL - 162
SP - 1689
EP - 1707
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 6
ER -