Advances in genosensor research

Kenneth L. Beattie; Wanda G. Beattie; Lin Meng; Saralinda L. Turner; Ramon Coral-Vazquez; Don D. Smith; Peter M. McIntyre; Dat D. Dao

doi:10.1093/clinchem/41.5.700

Advances in genosensor research

Kenneth L. Beattie, Wanda G. Beattie, Lin Meng, Saralinda L. Turner, Ramon Coral-Vazquez, Don D. Smith, Peter M. McIntyre, Dat D. Dao

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

Microfabricated devices containing arrays of nucleic acid hybridization sites, known as genosensors, are being developed for a variety of uses in genomic analysis. A great deal of the overall genosensor development effort involves optimization of experimental conditions in the actual use of genosensors. Here we describe a 'low-tech' form of genosensor technology, involving arrays of oligonucleotides on glass microscope slides, which can be used to define optimal operating conditions and to develop applications of hybridization arrays in genome mapping and sequencing. In addition, we describe a porous silicon genosensor, which can be operated in a flowthrough mode, and discuss its advantages over current flat-surface designs. Porous silicon genosensors containing arrays of DNA fragments offer several unique capabilities in genome analysis.

Original language	English
Pages (from-to)	700-706
Number of pages	7
Journal	Clinical Chemistry
Volume	41
Issue number	5
DOIs	https://doi.org/10.1093/clinchem/41.5.700
State	Published - 1995
Externally published	Yes

Keywords

DNA chips
DNA probes
DNA sequencing
genome mapping
nucleic acid hybridization
oligonucleotide arrays
porous silicon

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10.1093/clinchem/41.5.700

Cite this

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AU - Beattie, Wanda G.

AU - Meng, Lin

AU - Turner, Saralinda L.

AU - Coral-Vazquez, Ramon

AU - Smith, Don D.

AU - McIntyre, Peter M.

AU - Dao, Dat D.

PY - 1995

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Advances in genosensor research

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Keywords

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