TY - JOUR
T1 - Adjuvant immunotherapy of C6 glioma in rats with pertussis toxin
AU - Orozco-Morales, Mario
AU - Sánchez-García, Francisco Javier
AU - Guevara-Salazar, Patricia
AU - Arrieta, Oscar
AU - Hernández-Pedro, Norma Y.
AU - Sánchez-García, Aurora
AU - Perez-Madrigal, Rodolfo
AU - Rangel-López, Edgar
AU - Pineda, Benjamín
AU - Sotelo, Julio
N1 - Funding Information:
Acknowledgments This work was partially supported by CONA-CyT-México grants (90550) and ICyTDF (PICSA10-143).
PY - 2012/1
Y1 - 2012/1
N2 - Purpose In spite of the recent advances in surgery and antitumor drugs, the brain tumors, like glioblastoma, have shown a poor prognosis. The aim of this study was to determine the eVect of pertussis toxin (PTx) as immunomodulatory molecule on glial tumors induced by C6 glioma cells. Methods Given the pleiotropic eVect of PTx on the immune system, we analyzed the eVect of PTx on CD4+/ CD25+/FoxP3+ (Treg) cells like as immunotherapeutic adjuvant. Thirty rats with a glial tumor of 1.5 cm in diameter were separated in two groups: the Wrst group was treated with PTx and the second group was non-treated (controls). Tumoral volume was measured weekly; tumor, blood and spleen were taken for analysis of subpopulations of T cells, apoptotic index and cytokine contents, in both groups. Results We observed a signiWcant decrease in tumor volume in the PTx group; this was associated with a decreased in the number of Treg cells, in both spleen and tumor. The percentage of apoptotic cells was increased as compared with that of controls. The production of proinXammatory cytokines was increased in mRNA for IL-6 as well as a small increase in the mRNA expression of perforin and granzime in tumors from rats treated with PTx. No changes were found in the mRNA expression of MCP-1 and MIP-1α.Conclusion These results suggest that PTx could be an immunotherapeutic adjuvant in the integral therapy against glial tumors.
AB - Purpose In spite of the recent advances in surgery and antitumor drugs, the brain tumors, like glioblastoma, have shown a poor prognosis. The aim of this study was to determine the eVect of pertussis toxin (PTx) as immunomodulatory molecule on glial tumors induced by C6 glioma cells. Methods Given the pleiotropic eVect of PTx on the immune system, we analyzed the eVect of PTx on CD4+/ CD25+/FoxP3+ (Treg) cells like as immunotherapeutic adjuvant. Thirty rats with a glial tumor of 1.5 cm in diameter were separated in two groups: the Wrst group was treated with PTx and the second group was non-treated (controls). Tumoral volume was measured weekly; tumor, blood and spleen were taken for analysis of subpopulations of T cells, apoptotic index and cytokine contents, in both groups. Results We observed a signiWcant decrease in tumor volume in the PTx group; this was associated with a decreased in the number of Treg cells, in both spleen and tumor. The percentage of apoptotic cells was increased as compared with that of controls. The production of proinXammatory cytokines was increased in mRNA for IL-6 as well as a small increase in the mRNA expression of perforin and granzime in tumors from rats treated with PTx. No changes were found in the mRNA expression of MCP-1 and MIP-1α.Conclusion These results suggest that PTx could be an immunotherapeutic adjuvant in the integral therapy against glial tumors.
KW - C6 glioma
KW - Glioblastoma multiforme
KW - Immunotherapeutic
KW - Pertussis toxin
KW - Regulatory T cells
UR - http://www.scopus.com/inward/record.url?scp=84857064068&partnerID=8YFLogxK
U2 - 10.1007/s00432-011-1069-y
DO - 10.1007/s00432-011-1069-y
M3 - Artículo
SN - 0171-5216
VL - 138
SP - 23
EP - 33
JO - Journal of Cancer Research and Clinical Oncology
JF - Journal of Cancer Research and Clinical Oncology
IS - 1
ER -