TY - JOUR
T1 - Adaptive response induced by mitomycin C measuring the frequency of SCEs in human lymphocyte cultures
AU - Madrigal-Bujaidar, E.
AU - Cassani, M.
AU - Martínez, S.
AU - Morales, T.
PY - 1994/10
Y1 - 1994/10
N2 - The induction of an adaptive response was obtained using mitomycin C (MMC) as both stimulating and challenging agent. Human lymphocyte cultures of two female donors were treated with 5, 10 and 20 ng/ml of MMC as conditioning doses. For the challenging treatments two different protocols were used (200 ng/ml for 4 h, and 400 ng/ml for 1 h). The scoring of sister chromatid exchanges (SCE) in the first challenging combination showed the following inhibition related with the expected SCE damage: 49.2%, 51.4%, and 36.9% for one donor, and 42.0%, 38.6%, and 34.7% for the other (corresponding to the stimulating dosages 5, 10, and 20 ng/ml, respectively). The second challenging combination gave an inhibition of 53.8%, 40.5% and 30.2% in one donor and 43.2%, 45.9% and 30.3% in the other donor.
AB - The induction of an adaptive response was obtained using mitomycin C (MMC) as both stimulating and challenging agent. Human lymphocyte cultures of two female donors were treated with 5, 10 and 20 ng/ml of MMC as conditioning doses. For the challenging treatments two different protocols were used (200 ng/ml for 4 h, and 400 ng/ml for 1 h). The scoring of sister chromatid exchanges (SCE) in the first challenging combination showed the following inhibition related with the expected SCE damage: 49.2%, 51.4%, and 36.9% for one donor, and 42.0%, 38.6%, and 34.7% for the other (corresponding to the stimulating dosages 5, 10, and 20 ng/ml, respectively). The second challenging combination gave an inhibition of 53.8%, 40.5% and 30.2% in one donor and 43.2%, 45.9% and 30.3% in the other donor.
KW - Adaptive response
KW - Human lymphocyte
KW - Mitomycin C
KW - SCE
UR - http://www.scopus.com/inward/record.url?scp=0028108455&partnerID=8YFLogxK
U2 - 10.1016/0165-1218(94)90106-6
DO - 10.1016/0165-1218(94)90106-6
M3 - Artículo
SN - 0165-1218
VL - 322
SP - 301
EP - 305
JO - Mutation Research - Genetic Toxicology
JF - Mutation Research - Genetic Toxicology
IS - 4
ER -