TY - JOUR
T1 - Actin filaments and microtubule dual-granule transport in human adhered platelets
T2 - The role of α-dystrobrevins
AU - Cerecedo, Doris
AU - Cisneros, Bulmaro
AU - Mondragón, Ricardo
AU - González, Sirenia
AU - Galván, Iván J.
PY - 2010/4
Y1 - 2010/4
N2 - Upon activation with physiological stimuli, human platelets undergo morphological changes, centralizing their organelles and secreting effector molecules at the site of vascular injury. Previous studies have indicated that the actin filaments and microtubules of suspension-activated platelets play a critical role in granule movement and exocytosis; however, the participation of these cytoskeleton elements in adhered platelets remains unexplored. α- and β-dystrobrevin members of the dystrophin-associated protein complex in muscle and non-muscle cells have been described as motor protein receptors that might participate in the transport of cellular components in neurons. Recently, we characterized the expression of dystrobrevins in platelets; however, their functional diversity within this cellular model had not been elucidated. The present study examined the contribution of actin filaments and microtubules in granule trafficking during the platelet adhesion process using cytoskeleton-disrupting drugs, quantification of soluble P-selectin, fluorescence resonance transfer energy analysis and immunoprecipitation assays. Likewise, we assessed the interaction of α-dystrobrevins with the ubiquitous kinesin heavy chain. Our results strongly suggest that microtubules and actin filaments participate in the transport of alpha and dense granules in the platelet adhesion process, during which α-dystrobrevins play the role of regulatory and adaptor proteins that govern trafficking events.
AB - Upon activation with physiological stimuli, human platelets undergo morphological changes, centralizing their organelles and secreting effector molecules at the site of vascular injury. Previous studies have indicated that the actin filaments and microtubules of suspension-activated platelets play a critical role in granule movement and exocytosis; however, the participation of these cytoskeleton elements in adhered platelets remains unexplored. α- and β-dystrobrevin members of the dystrophin-associated protein complex in muscle and non-muscle cells have been described as motor protein receptors that might participate in the transport of cellular components in neurons. Recently, we characterized the expression of dystrobrevins in platelets; however, their functional diversity within this cellular model had not been elucidated. The present study examined the contribution of actin filaments and microtubules in granule trafficking during the platelet adhesion process using cytoskeleton-disrupting drugs, quantification of soluble P-selectin, fluorescence resonance transfer energy analysis and immunoprecipitation assays. Likewise, we assessed the interaction of α-dystrobrevins with the ubiquitous kinesin heavy chain. Our results strongly suggest that microtubules and actin filaments participate in the transport of alpha and dense granules in the platelet adhesion process, during which α-dystrobrevins play the role of regulatory and adaptor proteins that govern trafficking events.
KW - Actin-based structures
KW - Cytoskeleton remodelling
KW - Granule dispersion
KW - Granulomere
KW - Platelet adhesion
UR - http://www.scopus.com/inward/record.url?scp=77949422498&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2141.2010.08085.x
DO - 10.1111/j.1365-2141.2010.08085.x
M3 - Artículo
SN - 0007-1048
VL - 149
SP - 124
EP - 136
JO - British Journal of Haematology
JF - British Journal of Haematology
IS - 1
ER -