A simple silica-based method for metagenomic DNA extraction from soil and sediments

R. Rojas-Herrera; J. Narváez-Zapata; M. Zamudio-Maya; M. E. Mena-Martínez

doi:10.1007/s12033-008-9061-8

A simple silica-based method for metagenomic DNA extraction from soil and sediments

R. Rojas-Herrera, J. Narváez-Zapata, M. Zamudio-Maya, M. E. Mena-Martínez

Centro de Biotecnología Genómica (CBG)

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. High-quality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples.

Original language	English
Pages (from-to)	13-17
Number of pages	5
Journal	Molecular Biotechnology
Volume	40
Issue number	1
DOIs	https://doi.org/10.1007/s12033-008-9061-8
State	Published - Sep 2008

Keywords

DGGE
Metagenomic DNA
Microbial community
Sediments
Soils

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10.1007/s12033-008-9061-8

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abstract = "A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. High-quality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples.",

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AU - Rojas-Herrera, R.

AU - Narváez-Zapata, J.

AU - Zamudio-Maya, M.

AU - Mena-Martínez, M. E.

PY - 2008/9

Y1 - 2008/9

N2 - A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. High-quality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples.

AB - A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. High-quality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples.

KW - DGGE

KW - Metagenomic DNA

KW - Microbial community

KW - Sediments

KW - Soils

UR - http://www.scopus.com/inward/record.url?scp=55949087075&partnerID=8YFLogxK

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VL - 40

SP - 13

EP - 17

JO - Molecular Biotechnology

JF - Molecular Biotechnology

IS - 1

ER -

A simple silica-based method for metagenomic DNA extraction from soil and sediments

Abstract

Keywords

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