TY - JOUR
T1 - A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents
AU - Soto-Rojas, Luis O.
AU - Bañuelos, Cecilia
AU - Garces-Ramirez, Linda
AU - Luna-Herrera, Claudia
AU - Flores-Martínez, Yazmin M.
AU - Soto-Rodríguez, Guadalupe
AU - Gatica-García, Bismark
AU - López-Salas, Francisco E.
AU - Ayala-Dávila, José
AU - Gutiérrez-Castillo, María E.
AU - Padilla-Viveros, América
AU - de la Cruz-López, Fidel
AU - Martínez-Davila, Irma A.
AU - Martinez-Fong, Daniel
N1 - Publisher Copyright:
© 2020
PY - 2020
Y1 - 2020
N2 - An animal model, suitable for resembling Parkinson's disease (PD) progress, should show both, motor and non-motor alterations. However, these features have been scarcely evaluated or developed in parkinsonian models induced by neurotoxins. This protocol provides modifications to original methods, allowing six different motor and non-motor behavior tests, which adequately and timely emulate the main parkinsonian sensorimotor alterations in the rat or mouse: (1) bilateral sensorimotor alterations, examined by the vibrissae test; (2) balance and motor coordination, evaluated by the uncoordinated gait test; (3) locomotor asymmetry, analyzed by the cylinder test; (4) bradykinesia, as a locomotor alteration evidenced by the open field test; (5) depressive-like behavior, judged by the forced swimming test; and (6) hyposmia, assessed by the olfactory asymmetry test. Some advantages of using these behavioral tests over others include: • No sophisticated materials or equipment are required for their application and evaluation. • They are used in rodent models for parkinsonian research, but they can also be helpful for studying other movement disorders. • These tests can accurately discriminate the affected side from the healthy one, after unilateral injury of one hemisphere, resulting in sensorimotor, olfactory or locomotor asymmetry.
AB - An animal model, suitable for resembling Parkinson's disease (PD) progress, should show both, motor and non-motor alterations. However, these features have been scarcely evaluated or developed in parkinsonian models induced by neurotoxins. This protocol provides modifications to original methods, allowing six different motor and non-motor behavior tests, which adequately and timely emulate the main parkinsonian sensorimotor alterations in the rat or mouse: (1) bilateral sensorimotor alterations, examined by the vibrissae test; (2) balance and motor coordination, evaluated by the uncoordinated gait test; (3) locomotor asymmetry, analyzed by the cylinder test; (4) bradykinesia, as a locomotor alteration evidenced by the open field test; (5) depressive-like behavior, judged by the forced swimming test; and (6) hyposmia, assessed by the olfactory asymmetry test. Some advantages of using these behavioral tests over others include: • No sophisticated materials or equipment are required for their application and evaluation. • They are used in rodent models for parkinsonian research, but they can also be helpful for studying other movement disorders. • These tests can accurately discriminate the affected side from the healthy one, after unilateral injury of one hemisphere, resulting in sensorimotor, olfactory or locomotor asymmetry.
KW - 6-OHDA
KW - A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents
KW - BSSG
KW - Bradykinesia
KW - Depressive like-behavior
KW - Locomotor asymmetry
KW - Parkinsonism
KW - Sensorimotor alteration
KW - Uncoordinated gait
UR - http://www.scopus.com/inward/record.url?scp=85081310188&partnerID=8YFLogxK
U2 - 10.1016/j.mex.2020.100821
DO - 10.1016/j.mex.2020.100821
M3 - Artículo
C2 - 32195138
SN - 2215-0161
VL - 7
JO - MethodsX
JF - MethodsX
M1 - 100821
ER -