TY - JOUR
T1 - A red beet (Beta vulgaris) UDP-glucosyltransferase gene induced by wounding, bacterial infiltration and oxidative stress
AU - Sepúlveda-Jiménez, Gabriela
AU - Rueda-Benítez, Patricia
AU - Porta, Helena
AU - Rocha-Sosa, Mario
PY - 2005/2
Y1 - 2005/2
N2 - Mechanical wounding, infiltration with P. syringae or A. tumefaciens, and exposure to an H2O2-generating system (Glc/Glc oxidase) induce betacyanin synthesis in red beet (Beta vulgaris) leaves. These conditions also induced the expression of BvGT, a gene encoding a glucosyltransferase (GT) from Beta vulgaris. BvGT has a high similarity to Dorotheanthus bellidiformis betanidin-5 GT involved in betacyanin synthesis. Furthermore, the transient expression of a BvGT antisense construct resulted in the reduction of BvGT transcript accumulation and betanin synthesis, suggesting a role for this gene product in betacyanin glucosylation. In addition, the NADPH oxidase inhibitor, diphenylene iodonium (DPI), inhibited the accumulation of the BvGT transcript in response to infiltration with Agrobacterium tumefaciens. Hence, this result suggests that ROS produced by a plasma membrane NADPH oxidase may act as a signal to induce BvGT expression, necessary for betanin synthesis after wounding and bacterial infiltration.
AB - Mechanical wounding, infiltration with P. syringae or A. tumefaciens, and exposure to an H2O2-generating system (Glc/Glc oxidase) induce betacyanin synthesis in red beet (Beta vulgaris) leaves. These conditions also induced the expression of BvGT, a gene encoding a glucosyltransferase (GT) from Beta vulgaris. BvGT has a high similarity to Dorotheanthus bellidiformis betanidin-5 GT involved in betacyanin synthesis. Furthermore, the transient expression of a BvGT antisense construct resulted in the reduction of BvGT transcript accumulation and betanin synthesis, suggesting a role for this gene product in betacyanin glucosylation. In addition, the NADPH oxidase inhibitor, diphenylene iodonium (DPI), inhibited the accumulation of the BvGT transcript in response to infiltration with Agrobacterium tumefaciens. Hence, this result suggests that ROS produced by a plasma membrane NADPH oxidase may act as a signal to induce BvGT expression, necessary for betanin synthesis after wounding and bacterial infiltration.
KW - Bacterial infiltration
KW - Beta vulgaris
KW - Betacyanin
KW - Glucosyltransferase
KW - Red beet
KW - Wounding
UR - http://www.scopus.com/inward/record.url?scp=14544307566&partnerID=8YFLogxK
U2 - 10.1093/jxb/eri036
DO - 10.1093/jxb/eri036
M3 - Artículo
C2 - 15582929
SN - 0022-0957
VL - 56
SP - 605
EP - 611
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
IS - 412
ER -