A novel, rapid assay for detection and differentiation of serotype-specific antibodies to Venezuelan equine encephalitis complex alphaviruses

Eryu Wang; Slobodan Paessler; Patricia V. Aguilar; Darci R. Smith; Lark L. Coffey; Wenli Kang; Martin Pfeffer; James Olson; Patrick J. Blair; Carolina Guevara; Jose Estrada-Franco; Scott C. Weaver

doi:10.4269/ajtmh.2005.72.805

A novel, rapid assay for detection and differentiation of serotype-specific antibodies to Venezuelan equine encephalitis complex alphaviruses

Eryu Wang, Slobodan Paessler, Patricia V. Aguilar, Darci R. Smith, Lark L. Coffey, Wenli Kang, Martin Pfeffer, James Olson, Patrick J. Blair, Carolina Guevara, Jose Estrada-Franco, Scott C. Weaver

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

An epitope-blocking enzyme-linked immunosorbent assay was developed for the rapid differentiation of serologic responses to enzootic variety IE and ID versus epizootic variety IAB and IC strains of Venezuelan equine encephalitis (VEE) virus. Two monoclonal antibodies that differentially recognize epizootic versus enzootic VEE virus epitopes were used to measure the serotype-specific blocking abilities of antibodies in sera of naturally infected humans, equines, and bovines, as well as in experimentally infected equines. The assay is simple, species-independent, rapid, and sensitive, and will improve surveillance for VEE emergence. It could also be used to determine the epidemic potential of a VEE virus following an intentional introduction for bioterrorism.

Original language	English
Pages (from-to)	805-810
Number of pages	6
Journal	American Journal of Tropical Medicine and Hygiene
Volume	72
Issue number	6
DOIs	https://doi.org/10.4269/ajtmh.2005.72.805
State	Published - Jun 2005
Externally published	Yes

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10.4269/ajtmh.2005.72.805

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Wang, E., Paessler, S., Aguilar, P. V., Smith, D. R., Coffey, L. L., Kang, W., Pfeffer, M., Olson, J., Blair, P. J., Guevara, C., Estrada-Franco, J., & Weaver, S. C. (2005). A novel, rapid assay for detection and differentiation of serotype-specific antibodies to Venezuelan equine encephalitis complex alphaviruses. American Journal of Tropical Medicine and Hygiene, 72(6), 805-810. https://doi.org/10.4269/ajtmh.2005.72.805

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title = "A novel, rapid assay for detection and differentiation of serotype-specific antibodies to Venezuelan equine encephalitis complex alphaviruses",

abstract = "An epitope-blocking enzyme-linked immunosorbent assay was developed for the rapid differentiation of serologic responses to enzootic variety IE and ID versus epizootic variety IAB and IC strains of Venezuelan equine encephalitis (VEE) virus. Two monoclonal antibodies that differentially recognize epizootic versus enzootic VEE virus epitopes were used to measure the serotype-specific blocking abilities of antibodies in sera of naturally infected humans, equines, and bovines, as well as in experimentally infected equines. The assay is simple, species-independent, rapid, and sensitive, and will improve surveillance for VEE emergence. It could also be used to determine the epidemic potential of a VEE virus following an intentional introduction for bioterrorism.",

author = "Eryu Wang and Slobodan Paessler and Aguilar, {Patricia V.} and Smith, {Darci R.} and Coffey, {Lark L.} and Wenli Kang and Martin Pfeffer and James Olson and Blair, {Patrick J.} and Carolina Guevara and Jose Estrada-Franco and Weaver, {Scott C.}",

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Wang, E, Paessler, S, Aguilar, PV, Smith, DR, Coffey, LL, Kang, W, Pfeffer, M, Olson, J, Blair, PJ, Guevara, C, Estrada-Franco, J & Weaver, SC 2005, 'A novel, rapid assay for detection and differentiation of serotype-specific antibodies to Venezuelan equine encephalitis complex alphaviruses', American Journal of Tropical Medicine and Hygiene, vol. 72, no. 6, pp. 805-810. https://doi.org/10.4269/ajtmh.2005.72.805

TY - JOUR

T1 - A novel, rapid assay for detection and differentiation of serotype-specific antibodies to Venezuelan equine encephalitis complex alphaviruses

AU - Wang, Eryu

AU - Paessler, Slobodan

AU - Aguilar, Patricia V.

AU - Smith, Darci R.

AU - Coffey, Lark L.

AU - Kang, Wenli

AU - Pfeffer, Martin

AU - Olson, James

AU - Blair, Patrick J.

AU - Guevara, Carolina

AU - Estrada-Franco, Jose

AU - Weaver, Scott C.

PY - 2005/6

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AB - An epitope-blocking enzyme-linked immunosorbent assay was developed for the rapid differentiation of serologic responses to enzootic variety IE and ID versus epizootic variety IAB and IC strains of Venezuelan equine encephalitis (VEE) virus. Two monoclonal antibodies that differentially recognize epizootic versus enzootic VEE virus epitopes were used to measure the serotype-specific blocking abilities of antibodies in sera of naturally infected humans, equines, and bovines, as well as in experimentally infected equines. The assay is simple, species-independent, rapid, and sensitive, and will improve surveillance for VEE emergence. It could also be used to determine the epidemic potential of a VEE virus following an intentional introduction for bioterrorism.

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U2 - 10.4269/ajtmh.2005.72.805

DO - 10.4269/ajtmh.2005.72.805

M3 - Artículo

SN - 0002-9637

VL - 72

SP - 805

EP - 810

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

IS - 6

ER -

A novel, rapid assay for detection and differentiation of serotype-specific antibodies to Venezuelan equine encephalitis complex alphaviruses

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