TY - JOUR
T1 - A novel ADP/ATP transporter in the mitosome of the microaerophilic human parasite Entamoeba histolytica
AU - Chan, Ka Wai
AU - Slotboom, Dirk Jan
AU - Cox, Sian
AU - Embley, T. Martin
AU - Fabre, Olivier
AU - Van Der Giezen, Mark
AU - Harding, Marilyn
AU - Horner, David S.
AU - Kunji, Edmund R.S.
AU - León-Avila, Gloria
AU - Tovar, Jorge
N1 - Funding Information:
Preliminary genomic sequence data for Entamoeba were obtained from the Sanger Institute at ftp://ftp.sanger.ac.uk/pub/pathogens/E_histolytica and the Institute for Genomic Research at http://www.tigr.org . Sequencing of the TIGR Entamoeba histolytica Genome Project was accomplished with support from the National Institute of Allergy and Infectious Diseases (NIAID). D.J.S. was supported by a long-term fellowship of the International Human Frontier Science Program. K.W.C., M.H., and E.R.S.K. were supported by the Medical Research Council. E.R.S.K. acknowledges the support of the European Molecular Biology Organisation (EMBO) Young Investigators Programme. M.v.d.G. was supported in part by the Leverhulme Trust. S.C. acknowledges support from the Nuffield Foundation. Research at Royal Holloway was supported by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC) to J.T.
PY - 2005/4/26
Y1 - 2005/4/26
N2 - Recent data suggest that microaerophilic and parasitic protozoa, which lack oxidative phosphorylation, nevertheless contain mitochondrial homologs [1-6], organelles that share common ancestry with mitochondria. Such widespread retention suggests there may be a common function for mitochondrial homologs that makes them essential for eukaryotic cells. We determined the mitochondrial carrier family (MCF) complement of the Entamoeba histolytica mitochondrial homolog, also known as a crypton [5] or more commonly as a mitosome [3]. MCF proteins support mitochondrial metabolic energy generation, DNA replication, and amino-acid metabolism by linking biochemical pathways in the mitochondrial matrix with those in the cytosol [7]. MCF diversity thus closely mirrors important facets of mitochondrial metabolic diversity. The Entamoeba histolytica mitosome has lost all but a single type of MCF protein, which transports ATP and ADP via a novel mechanism that is not reliant on a membrane potential. Phylogenetic analyses confirm that the Entamoeba ADP/ATP carrier is distinct from archetypal mitochondrial ADP/ATP carriers, an observation that is supported by its different substrate and inhibitor specificity. Because many functions of yeast and human mitochondria rely on solutes transported by specialized members of this family, the Entamoeba mitosome must contain only a small subset of these processes requiring adenine nucleotide exchange.
AB - Recent data suggest that microaerophilic and parasitic protozoa, which lack oxidative phosphorylation, nevertheless contain mitochondrial homologs [1-6], organelles that share common ancestry with mitochondria. Such widespread retention suggests there may be a common function for mitochondrial homologs that makes them essential for eukaryotic cells. We determined the mitochondrial carrier family (MCF) complement of the Entamoeba histolytica mitochondrial homolog, also known as a crypton [5] or more commonly as a mitosome [3]. MCF proteins support mitochondrial metabolic energy generation, DNA replication, and amino-acid metabolism by linking biochemical pathways in the mitochondrial matrix with those in the cytosol [7]. MCF diversity thus closely mirrors important facets of mitochondrial metabolic diversity. The Entamoeba histolytica mitosome has lost all but a single type of MCF protein, which transports ATP and ADP via a novel mechanism that is not reliant on a membrane potential. Phylogenetic analyses confirm that the Entamoeba ADP/ATP carrier is distinct from archetypal mitochondrial ADP/ATP carriers, an observation that is supported by its different substrate and inhibitor specificity. Because many functions of yeast and human mitochondria rely on solutes transported by specialized members of this family, the Entamoeba mitosome must contain only a small subset of these processes requiring adenine nucleotide exchange.
UR - http://www.scopus.com/inward/record.url?scp=18044388434&partnerID=8YFLogxK
U2 - 10.1016/j.cub.2005.02.068
DO - 10.1016/j.cub.2005.02.068
M3 - Artículo
C2 - 15854906
AN - SCOPUS:18044388434
SN - 0960-9822
VL - 15
SP - 737
EP - 742
JO - Current Biology
JF - Current Biology
IS - 8
ER -