TY - JOUR
T1 - A new formulated stable papin-pectin aerosol spray for skin wound healing
AU - Jáuregui, Karla Monzerratt Gregorio
AU - Cabrera, Juan Carlos Cano
AU - Ceniceros, Elda Patricia Segura
AU - Hernández, Jose Luis Martínez
AU - Ilyina, Anna
N1 - Funding Information:
^ÅâåçïäÉÇÖÉãÉåíë The authors are grateful for the financial support of this project from the Fondo Sectorial de Salud and Seguridad Social-CONACYT (SALUD-2005-01-13983) and the valuab le help of Siong N. G. for reviewing this paper for pub lication.
PY - 2009
Y1 - 2009
N2 - A new spray-on topical wound debrider composition consisting of 0.1% w/v papain immobilized in 6% w/v pectin gel was formulated for skin wound healing. The stability of the enzyme activity of this new formulated spray was compared with the 0.1% w/v papain in water solution at the refrigerated temperature of 4 and 75°C. Two aerosol propellants, air and nitrogen, were evaluated for their efficacy. The new formulated spray was then evaluated for its thermal stability under air and N2 at 75°C. All enzyme activity of papain was measured with a spectrophotometer using casein as a substrate at 37°C and in 0.1 M phosphate buffer at pH 6, as well as trichloral acetic acid to separate the product of casein hydrolysis. All the evaluations showed the new aerosol spray formulation to be far better than the liquid formulation in storage stability and thermal stability at 75°C under atmospheric or higher pressures. The new formulation was tested in a rabbit experiment to evaluate the effectiveness of wound healing. As compared to untreated wound, the formulated spray clearly was superior, yielding a 20% more healing progress in the first 4 days alone.
AB - A new spray-on topical wound debrider composition consisting of 0.1% w/v papain immobilized in 6% w/v pectin gel was formulated for skin wound healing. The stability of the enzyme activity of this new formulated spray was compared with the 0.1% w/v papain in water solution at the refrigerated temperature of 4 and 75°C. Two aerosol propellants, air and nitrogen, were evaluated for their efficacy. The new formulated spray was then evaluated for its thermal stability under air and N2 at 75°C. All enzyme activity of papain was measured with a spectrophotometer using casein as a substrate at 37°C and in 0.1 M phosphate buffer at pH 6, as well as trichloral acetic acid to separate the product of casein hydrolysis. All the evaluations showed the new aerosol spray formulation to be far better than the liquid formulation in storage stability and thermal stability at 75°C under atmospheric or higher pressures. The new formulation was tested in a rabbit experiment to evaluate the effectiveness of wound healing. As compared to untreated wound, the formulated spray clearly was superior, yielding a 20% more healing progress in the first 4 days alone.
KW - Papain stability
KW - Pectin gel
KW - Propellant pressure effect
UR - http://www.scopus.com/inward/record.url?scp=70349643554&partnerID=8YFLogxK
U2 - 10.1007/s12257-008-0268-0
DO - 10.1007/s12257-008-0268-0
M3 - Artículo
AN - SCOPUS:70349643554
SN - 1226-8372
VL - 14
SP - 450
EP - 456
JO - Biotechnology and Bioprocess Engineering
JF - Biotechnology and Bioprocess Engineering
IS - 4
ER -