TY - JOUR
T1 - A high-throughput colorimetric and fluorometric microassay for the evaluation of nitroimidazole derivatives anti-trichomonas activity
AU - Campos Aldrete, Ma Elena
AU - Salgado-Zamora, Héctor
AU - Luna, Julieta
AU - Meléndez, Estela
AU - Meráz-Ríos, Marco A.
N1 - Funding Information:
One of us Ma.E. Campos gratefully acknowledges the SUPERA program (ANUIES-Mexico) for financial support through Grant No. 4447.
PY - 2005/12
Y1 - 2005/12
N2 - Several nitroimidazole derivatives were synthesized and tested as possible trichomonicidal agents. A fast, simple, practical and reliable in vitro colorimetric method was applied to the screening of the nitroimidazole derivatives anti-trichomonas activity. The colorimetric technique was based on the use of Alamar blue as a redox-indicator. The test was carried out both qualitatively (minimal inhibitory concentration determined by naked eye observation) and quantitatively (fluorometric determination of 50% and 90% inhibitory concentrations), the latter took advantage of the dye fluorometric properties. The performance of the method was excellent affording an exactitude ≥97.86% and a reproducibility of 95% and no interference of the trichomonas-culture medium was observed during the test. Some of the nitroimidazole compounds tested showed a fair trichomonicidal activity, however none of them was as active as the model compound, metronidazole.
AB - Several nitroimidazole derivatives were synthesized and tested as possible trichomonicidal agents. A fast, simple, practical and reliable in vitro colorimetric method was applied to the screening of the nitroimidazole derivatives anti-trichomonas activity. The colorimetric technique was based on the use of Alamar blue as a redox-indicator. The test was carried out both qualitatively (minimal inhibitory concentration determined by naked eye observation) and quantitatively (fluorometric determination of 50% and 90% inhibitory concentrations), the latter took advantage of the dye fluorometric properties. The performance of the method was excellent affording an exactitude ≥97.86% and a reproducibility of 95% and no interference of the trichomonas-culture medium was observed during the test. Some of the nitroimidazole compounds tested showed a fair trichomonicidal activity, however none of them was as active as the model compound, metronidazole.
KW - Alamar blue
KW - Colorimetric method
KW - Metronidazole
KW - Nitroimidazole derivatives
KW - Trichomonicidal activity
UR - http://www.scopus.com/inward/record.url?scp=27644577601&partnerID=8YFLogxK
U2 - 10.1016/j.tiv.2005.04.007
DO - 10.1016/j.tiv.2005.04.007
M3 - Artículo
C2 - 15963680
SN - 0887-2333
VL - 19
SP - 1045
EP - 1050
JO - Toxicology in Vitro
JF - Toxicology in Vitro
IS - 8
ER -