TY - JOUR
T1 - A global screening assay to select for maize phenotypes with a high tolerance or resistance to fusarium verticillioides (Sacc.) nirenberg rots
AU - Román, Shamir Gabriel
AU - Quiroz-Chávez, Jesús
AU - Villalobos, Miguel
AU - Urías-Gutiérrez, Vianey
AU - Nava-Pérez, Eusebio
AU - Ruíz-May, Eliel
AU - Singh, Rupesh Kumar
AU - Sharma, Lav
AU - Quiroz-Figueroa, Francisco Roberto
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. article distributed under the terms and conditions of the Cre.
PY - 2020/12
Y1 - 2020/12
N2 - Fusarium verticillioides (Sacc.) Nirenberg (Fv) causes rots in maize around the world and produces mycotoxins that contaminate grains, making this species a significant health concern for both animals and humans. One of the best approaches to address rots is to identify highly tolerant or resistant genotypes that can be used for genetic improvement. The aim of the study was to evaluate dose-response assays to tolerance or resistance for Fv rots throughout the maize life cycle. These tests assessed the effects of Fv during post-germination development and the seedling (V2) stage by seed infection, the plantlet (V4) stage by substrate infection, and in the reproductive phase in maize stalks (R2 stage) and ears (R6 stage) by R1 stage inoculation. In all assays, the doses were effective at distinguishing contrasting phenotypes. Severity, root fresh weight, and aerial length were the most informative parameters at the V2 and V4 stages. Evaluation of the stalk necrosis area between and within the internodes of susceptible genotypes revealed significant differences among doses, and a positive correlation between necrosis and conidia concentration was observed in internodes. Injecting eight million conidia in the ear was sufficient for selecting different phenotypes. A total of 85% of the genotypes conserved their same capacity to respond to Fv infection throughout the maize life cycle, so that screening at the early vegetative stage (e.g., V2) could be useful for distinguishing contrasting phenotypes in the reproductive stage. Implementing these screening assays in a maize breeding program could be valuable for classifying the degrees of resilience of maize germplasms to Fv rots. This global screening has the potential to be employed to select against other Fusarium species.
AB - Fusarium verticillioides (Sacc.) Nirenberg (Fv) causes rots in maize around the world and produces mycotoxins that contaminate grains, making this species a significant health concern for both animals and humans. One of the best approaches to address rots is to identify highly tolerant or resistant genotypes that can be used for genetic improvement. The aim of the study was to evaluate dose-response assays to tolerance or resistance for Fv rots throughout the maize life cycle. These tests assessed the effects of Fv during post-germination development and the seedling (V2) stage by seed infection, the plantlet (V4) stage by substrate infection, and in the reproductive phase in maize stalks (R2 stage) and ears (R6 stage) by R1 stage inoculation. In all assays, the doses were effective at distinguishing contrasting phenotypes. Severity, root fresh weight, and aerial length were the most informative parameters at the V2 and V4 stages. Evaluation of the stalk necrosis area between and within the internodes of susceptible genotypes revealed significant differences among doses, and a positive correlation between necrosis and conidia concentration was observed in internodes. Injecting eight million conidia in the ear was sufficient for selecting different phenotypes. A total of 85% of the genotypes conserved their same capacity to respond to Fv infection throughout the maize life cycle, so that screening at the early vegetative stage (e.g., V2) could be useful for distinguishing contrasting phenotypes in the reproductive stage. Implementing these screening assays in a maize breeding program could be valuable for classifying the degrees of resilience of maize germplasms to Fv rots. This global screening has the potential to be employed to select against other Fusarium species.
KW - Fusariosis
KW - Maize breeding
KW - Zea mays L
UR - http://www.scopus.com/inward/record.url?scp=85109112423&partnerID=8YFLogxK
U2 - 10.3390/agronomy10121990
DO - 10.3390/agronomy10121990
M3 - Artículo
AN - SCOPUS:85109112423
SN - 2073-4395
VL - 10
JO - Agronomy
JF - Agronomy
IS - 12
M1 - 1990
ER -