TY - JOUR
T1 - A DNA probe specific for Aeromonas colonies
AU - Chacón, M. R.
AU - Castro-Escarpulli, G.
AU - Soler, L.
AU - Guarro, J.
AU - Figueras, M. J.
N1 - Funding Information:
This work has been supported by the grants: FIS 99/0944 from the Spanish Ministry of Health, from Fundació Ciència i Salut and by a fellowship PSR/99–01 from the Universitat Rovira i Virgili. We would like to thank the Colección Española de Cultivos Tipo for kindly providing isolates.
PY - 2002/11/1
Y1 - 2002/11/1
N2 - Members of the genus Aeromonas are important enteropathogens. Commercial identification systems are often unable to correctly identify Aeromonas strains and misidentification as Vibrio spp. is common. A digoxigenin-DNA probe based on a 237 bp of the glycerophospholipid-cholesterol acyltransferase gene has been tested in a colony hybridization assay. The probe hybridized with all Aeromonas species tested (n = 16) but not with strains of other enteropathogenic bacteria (n = 20). The probe allowed the unequivocal identification of Aeromonas in primary isolation media within 36 h.
AB - Members of the genus Aeromonas are important enteropathogens. Commercial identification systems are often unable to correctly identify Aeromonas strains and misidentification as Vibrio spp. is common. A digoxigenin-DNA probe based on a 237 bp of the glycerophospholipid-cholesterol acyltransferase gene has been tested in a colony hybridization assay. The probe hybridized with all Aeromonas species tested (n = 16) but not with strains of other enteropathogenic bacteria (n = 20). The probe allowed the unequivocal identification of Aeromonas in primary isolation media within 36 h.
UR - http://www.scopus.com/inward/record.url?scp=0036867380&partnerID=8YFLogxK
U2 - 10.1016/S0732-8893(02)00455-8
DO - 10.1016/S0732-8893(02)00455-8
M3 - Artículo
SN - 0732-8893
VL - 44
SP - 221
EP - 225
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 3
ER -