TY - JOUR
T1 - 17Β-Estradiol induces cyto-genotoxicity on blood cells of common carp (Cyprinus carpio)
AU - Orozco-Hernández, Luis
AU - Gutiérrez-Gómez, Adriana Andrea
AU - SanJuan-Reyes, Nely
AU - Islas-Flores, Hariz
AU - García-Medina, Sandra
AU - Galar-Martínez, Marcela
AU - Dublán-García, Octavio
AU - Natividad, Reyna
AU - Gómez-Oliván, Leobardo Manuel
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2018
Y1 - 2018
N2 - 17β-Estradiol, a natural hormone present at high concentrations in aquatic ecosystems, affects and modifies endocrine function in animals. In recent years research workers have expressed concern over its potential effects on aquatic organisms; however, little is known about its capacity to induce genetic damage or the pro-apoptotic effects of such damage on fish. Therefore, this study aimed to evaluate 17β-estradiol-induced cyto-genotoxicity in blood cells of the common carp Cyprinus carpio exposed to different concentrations (1 ng, 1 μg and 1 mg L−1). Peripheral blood samples were collected and evaluated by comet assay, micronucleus test, determination of caspase-3 activity and TUNEL assay at 12, 24, 48, 72 and 96 h of exposure. Increases in frequency of micronuclei, TUNEL-positive cells and caspase-3 activity were observed, particularly at the highest concentration. In contrast, the comet assay detected significant increases at 24 and 96 h with the 1 μg and 1 ng L−1 concentrations respectively. The set of assays used in the present study constitutes a reliable early warning biomarker for evaluating the toxicity induced by this type of emerging contaminants on aquatic species.
AB - 17β-Estradiol, a natural hormone present at high concentrations in aquatic ecosystems, affects and modifies endocrine function in animals. In recent years research workers have expressed concern over its potential effects on aquatic organisms; however, little is known about its capacity to induce genetic damage or the pro-apoptotic effects of such damage on fish. Therefore, this study aimed to evaluate 17β-estradiol-induced cyto-genotoxicity in blood cells of the common carp Cyprinus carpio exposed to different concentrations (1 ng, 1 μg and 1 mg L−1). Peripheral blood samples were collected and evaluated by comet assay, micronucleus test, determination of caspase-3 activity and TUNEL assay at 12, 24, 48, 72 and 96 h of exposure. Increases in frequency of micronuclei, TUNEL-positive cells and caspase-3 activity were observed, particularly at the highest concentration. In contrast, the comet assay detected significant increases at 24 and 96 h with the 1 μg and 1 ng L−1 concentrations respectively. The set of assays used in the present study constitutes a reliable early warning biomarker for evaluating the toxicity induced by this type of emerging contaminants on aquatic species.
KW - 17 β-Estradiol
KW - Caspase-3
KW - Comet assay
KW - Cyprinus carpio
KW - Micronucleus test
KW - TUNEL
UR - http://www.scopus.com/inward/record.url?scp=85030995506&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2017.10.030
DO - 10.1016/j.chemosphere.2017.10.030
M3 - Artículo
C2 - 29031051
SN - 0045-6535
VL - 191
SP - 118
EP - 127
JO - Chemosphere
JF - Chemosphere
ER -