17Β-Estradiol induces cyto-genotoxicity on blood cells of common carp (Cyprinus carpio)

Luis Orozco-Hernández, Adriana Andrea Gutiérrez-Gómez, Nely SanJuan-Reyes, Hariz Islas-Flores, Sandra García-Medina, Marcela Galar-Martínez, Octavio Dublán-García, Reyna Natividad, Leobardo Manuel Gómez-Oliván

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Abstract

© 2017 Elsevier Ltd 17β-Estradiol, a natural hormone present at high concentrations in aquatic ecosystems, affects and modifies endocrine function in animals. In recent years research workers have expressed concern over its potential effects on aquatic organisms; however, little is known about its capacity to induce genetic damage or the pro-apoptotic effects of such damage on fish. Therefore, this study aimed to evaluate 17β-estradiol-induced cyto-genotoxicity in blood cells of the common carp Cyprinus carpio exposed to different concentrations (1 ng, 1 μg and 1 mg L−1). Peripheral blood samples were collected and evaluated by comet assay, micronucleus test, determination of caspase-3 activity and TUNEL assay at 12, 24, 48, 72 and 96 h of exposure. Increases in frequency of micronuclei, TUNEL-positive cells and caspase-3 activity were observed, particularly at the highest concentration. In contrast, the comet assay detected significant increases at 24 and 96 h with the 1 μg and 1 ng L−1 concentrations respectively. The set of assays used in the present study constitutes a reliable early warning biomarker for evaluating the toxicity induced by this type of emerging contaminants on aquatic species.
Original languageAmerican English
Pages (from-to)118-127
Number of pages105
JournalChemosphere
DOIs
StatePublished - 1 Jan 2018

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Comet Assay
Carps
genotoxicity
In Situ Nick-End Labeling
Caspase 3
Estradiol
Assays
Blood Cells
Blood
blood
Cells
assay
Aquatic Organisms
Micronucleus Tests
comet
Ecosystem
Fishes
Biomarkers
Hormones
Aquatic organisms

Cite this

Orozco-Hernández, L., Gutiérrez-Gómez, A. A., SanJuan-Reyes, N., Islas-Flores, H., García-Medina, S., Galar-Martínez, M., ... Gómez-Oliván, L. M. (2018). 17Β-Estradiol induces cyto-genotoxicity on blood cells of common carp (Cyprinus carpio). Chemosphere, 118-127. https://doi.org/10.1016/j.chemosphere.2017.10.030
Orozco-Hernández, Luis ; Gutiérrez-Gómez, Adriana Andrea ; SanJuan-Reyes, Nely ; Islas-Flores, Hariz ; García-Medina, Sandra ; Galar-Martínez, Marcela ; Dublán-García, Octavio ; Natividad, Reyna ; Gómez-Oliván, Leobardo Manuel. / 17Β-Estradiol induces cyto-genotoxicity on blood cells of common carp (Cyprinus carpio). In: Chemosphere. 2018 ; pp. 118-127.
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abstract = "{\circledC} 2017 Elsevier Ltd 17β-Estradiol, a natural hormone present at high concentrations in aquatic ecosystems, affects and modifies endocrine function in animals. In recent years research workers have expressed concern over its potential effects on aquatic organisms; however, little is known about its capacity to induce genetic damage or the pro-apoptotic effects of such damage on fish. Therefore, this study aimed to evaluate 17β-estradiol-induced cyto-genotoxicity in blood cells of the common carp Cyprinus carpio exposed to different concentrations (1 ng, 1 μg and 1 mg L−1). Peripheral blood samples were collected and evaluated by comet assay, micronucleus test, determination of caspase-3 activity and TUNEL assay at 12, 24, 48, 72 and 96 h of exposure. Increases in frequency of micronuclei, TUNEL-positive cells and caspase-3 activity were observed, particularly at the highest concentration. In contrast, the comet assay detected significant increases at 24 and 96 h with the 1 μg and 1 ng L−1 concentrations respectively. The set of assays used in the present study constitutes a reliable early warning biomarker for evaluating the toxicity induced by this type of emerging contaminants on aquatic species.",
author = "Luis Orozco-Hern{\'a}ndez and Guti{\'e}rrez-G{\'o}mez, {Adriana Andrea} and Nely SanJuan-Reyes and Hariz Islas-Flores and Sandra Garc{\'i}a-Medina and Marcela Galar-Mart{\'i}nez and Octavio Dubl{\'a}n-Garc{\'i}a and Reyna Natividad and G{\'o}mez-Oliv{\'a}n, {Leobardo Manuel}",
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Orozco-Hernández, L, Gutiérrez-Gómez, AA, SanJuan-Reyes, N, Islas-Flores, H, García-Medina, S, Galar-Martínez, M, Dublán-García, O, Natividad, R & Gómez-Oliván, LM 2018, '17Β-Estradiol induces cyto-genotoxicity on blood cells of common carp (Cyprinus carpio)', Chemosphere, pp. 118-127. https://doi.org/10.1016/j.chemosphere.2017.10.030

17Β-Estradiol induces cyto-genotoxicity on blood cells of common carp (Cyprinus carpio). / Orozco-Hernández, Luis; Gutiérrez-Gómez, Adriana Andrea; SanJuan-Reyes, Nely; Islas-Flores, Hariz; García-Medina, Sandra; Galar-Martínez, Marcela; Dublán-García, Octavio; Natividad, Reyna; Gómez-Oliván, Leobardo Manuel.

In: Chemosphere, 01.01.2018, p. 118-127.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - 17Β-Estradiol induces cyto-genotoxicity on blood cells of common carp (Cyprinus carpio)

AU - Orozco-Hernández, Luis

AU - Gutiérrez-Gómez, Adriana Andrea

AU - SanJuan-Reyes, Nely

AU - Islas-Flores, Hariz

AU - García-Medina, Sandra

AU - Galar-Martínez, Marcela

AU - Dublán-García, Octavio

AU - Natividad, Reyna

AU - Gómez-Oliván, Leobardo Manuel

PY - 2018/1/1

Y1 - 2018/1/1

N2 - © 2017 Elsevier Ltd 17β-Estradiol, a natural hormone present at high concentrations in aquatic ecosystems, affects and modifies endocrine function in animals. In recent years research workers have expressed concern over its potential effects on aquatic organisms; however, little is known about its capacity to induce genetic damage or the pro-apoptotic effects of such damage on fish. Therefore, this study aimed to evaluate 17β-estradiol-induced cyto-genotoxicity in blood cells of the common carp Cyprinus carpio exposed to different concentrations (1 ng, 1 μg and 1 mg L−1). Peripheral blood samples were collected and evaluated by comet assay, micronucleus test, determination of caspase-3 activity and TUNEL assay at 12, 24, 48, 72 and 96 h of exposure. Increases in frequency of micronuclei, TUNEL-positive cells and caspase-3 activity were observed, particularly at the highest concentration. In contrast, the comet assay detected significant increases at 24 and 96 h with the 1 μg and 1 ng L−1 concentrations respectively. The set of assays used in the present study constitutes a reliable early warning biomarker for evaluating the toxicity induced by this type of emerging contaminants on aquatic species.

AB - © 2017 Elsevier Ltd 17β-Estradiol, a natural hormone present at high concentrations in aquatic ecosystems, affects and modifies endocrine function in animals. In recent years research workers have expressed concern over its potential effects on aquatic organisms; however, little is known about its capacity to induce genetic damage or the pro-apoptotic effects of such damage on fish. Therefore, this study aimed to evaluate 17β-estradiol-induced cyto-genotoxicity in blood cells of the common carp Cyprinus carpio exposed to different concentrations (1 ng, 1 μg and 1 mg L−1). Peripheral blood samples were collected and evaluated by comet assay, micronucleus test, determination of caspase-3 activity and TUNEL assay at 12, 24, 48, 72 and 96 h of exposure. Increases in frequency of micronuclei, TUNEL-positive cells and caspase-3 activity were observed, particularly at the highest concentration. In contrast, the comet assay detected significant increases at 24 and 96 h with the 1 μg and 1 ng L−1 concentrations respectively. The set of assays used in the present study constitutes a reliable early warning biomarker for evaluating the toxicity induced by this type of emerging contaminants on aquatic species.

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