TY - JOUR
T1 - Virulence factors and antimicrobial resistance in environmental strains of Vibrio alginolyticus
AU - Hernández-Robles, Marcos F.
AU - Álvarez-Contreras, Ana K.
AU - Juárez-García, Patricia
AU - Natividad-Bonifacio, Iván
AU - Curiel-Quesada, Everardo
AU - Vázquez-Salinas, Carlos
AU - Quiñones-Ramírez, Elsa Irma
N1 - Publisher Copyright:
© 2016, Sociedad Espanola de Microbiologia. All rights reserved.
PY - 2016
Y1 - 2016
N2 - Vibrio alginolyticus has acquired increasing importance because this microorganism may be pathogenic to aquatic animals and humans. It has been reported that some V. alginolyticus strains carry virulence genes derived from pathogenic V. cholerae and V. parahaemolyticus strains. In this work V. alginolyticus was isolated from oyster samples acquired from a food-market in Mexico City. Thirty isolates were identified as V. alginolitycus. Strains showed β-haemolysis and proteolytic activity and produced a capsule. Strains displayed swimming and swarming motility and 93.3% of them produced siderophores. Several genes encoding virulence factors were detected using PCR amplification. These included proA, wza, vopD, vopB, hcp, vasH and vgrG genes, which were present in all strains. Other genes had a variable representation: tdh (86.6%), lafA (96.6%), pvsA (62%) and pvuA (16%). The trh gene could not be amplified from any of the strains. The antimicrobial resistance profile revealed that more than 90% of the strains were resistant to beta-lactams antibiotics, 60% to cephalotin, 45% to amikacin, 16% to cephotaxime, and 10% to pefloxacin, while 100% were susceptible to ceftriaxone. The V. alginolyticus strains isolated from oysters showed multiple resistance to antibiotics and several virulence factors described in well-characterized pathogenic vibrios.
AB - Vibrio alginolyticus has acquired increasing importance because this microorganism may be pathogenic to aquatic animals and humans. It has been reported that some V. alginolyticus strains carry virulence genes derived from pathogenic V. cholerae and V. parahaemolyticus strains. In this work V. alginolyticus was isolated from oyster samples acquired from a food-market in Mexico City. Thirty isolates were identified as V. alginolitycus. Strains showed β-haemolysis and proteolytic activity and produced a capsule. Strains displayed swimming and swarming motility and 93.3% of them produced siderophores. Several genes encoding virulence factors were detected using PCR amplification. These included proA, wza, vopD, vopB, hcp, vasH and vgrG genes, which were present in all strains. Other genes had a variable representation: tdh (86.6%), lafA (96.6%), pvsA (62%) and pvuA (16%). The trh gene could not be amplified from any of the strains. The antimicrobial resistance profile revealed that more than 90% of the strains were resistant to beta-lactams antibiotics, 60% to cephalotin, 45% to amikacin, 16% to cephotaxime, and 10% to pefloxacin, while 100% were susceptible to ceftriaxone. The V. alginolyticus strains isolated from oysters showed multiple resistance to antibiotics and several virulence factors described in well-characterized pathogenic vibrios.
KW - Capsular polyssacharides
KW - Oysters
KW - Secretion system
KW - Vibrio alginolyticus
KW - Virulence factors
UR - http://www.scopus.com/inward/record.url?scp=85019603942&partnerID=8YFLogxK
U2 - 10.2436/20.1501.01.277
DO - 10.2436/20.1501.01.277
M3 - Artículo
SN - 1139-6709
VL - 19
SP - 191
EP - 198
JO - International Microbiology
JF - International Microbiology
IS - 4
ER -