TY - JOUR
T1 - Tropane alkaloid production in Datura stramonium root cultures
AU - Maldonado-Mendoza, Ignacio E.
AU - Del Ayora-Talavera, Terasa R.
AU - Loyola-Vargas, Victor M.
PY - 1992/4
Y1 - 1992/4
N2 - Tropane alkaloid production was studied in different root cultures of Datura stramonium. Cultured roots were obtained with 10-6 M of indolbutyric acid. Their doubling times were from 6 to 19 days. Hyoscyamine content varied from 0.17 to 0.62% dry weight, and scopolamine content from 0.08 to 0.33% dry weight, depending on the lines. A comparison of the bioproductivity of these compounds in the pot-grown plant roots showed that it was two to three orders lower than cultured roots, and it increased one order of magnitude considering the productivity on the whole plant. Bioproductivity, growth capacity and alkaloid production stability during subsequent transfers (more than 2 yr) are reported. Only one root line (N5) showed excretion of the alkaloids to the culture medium. Characterization of three selected lines (N1, N5, and N9) showed that the highest alkaloid production is reached at the stationary phase of growth, with the exception of line N9.
AB - Tropane alkaloid production was studied in different root cultures of Datura stramonium. Cultured roots were obtained with 10-6 M of indolbutyric acid. Their doubling times were from 6 to 19 days. Hyoscyamine content varied from 0.17 to 0.62% dry weight, and scopolamine content from 0.08 to 0.33% dry weight, depending on the lines. A comparison of the bioproductivity of these compounds in the pot-grown plant roots showed that it was two to three orders lower than cultured roots, and it increased one order of magnitude considering the productivity on the whole plant. Bioproductivity, growth capacity and alkaloid production stability during subsequent transfers (more than 2 yr) are reported. Only one root line (N5) showed excretion of the alkaloids to the culture medium. Characterization of three selected lines (N1, N5, and N9) showed that the highest alkaloid production is reached at the stationary phase of growth, with the exception of line N9.
KW - Datura stramonium
KW - hyoscyamine
KW - root culture
KW - scopolamine
KW - tropane alkaloids
UR - http://www.scopus.com/inward/record.url?scp=51649135151&partnerID=8YFLogxK
U2 - 10.1007/BF02823021
DO - 10.1007/BF02823021
M3 - Artículo
AN - SCOPUS:51649135151
SN - 1054-5476
VL - 28
SP - 67
EP - 72
JO - In Vitro Cellular and Developmental Biology - Plant
JF - In Vitro Cellular and Developmental Biology - Plant
IS - 2
ER -