TY - JOUR
T1 - Toxic effect of titanium dioxide nanoparticles on human mesenchymal stem cells
AU - Adriana-Berenice, Peralta Vega
AU - Alberto, Parra Barrera
AU - del Pilar, Ramos Godínez María
AU - Rebeca, López Marure
AU - José, Arellano Galindo
AU - Gutiérrez-Iglesias, Gisela
N1 - Publisher Copyright:
© 2020, The Korean Society of Toxicogenomics and Toxicoproteomics 2020.
PY - 2020/7/1
Y1 - 2020/7/1
N2 - Background: Titanium dioxide (TiO2) is employed in the production of cosmetics, sunscreen, food, and drugs; however, TiO2 is toxic at the nanometric scale. Objective: To analyze the in vitro toxic effect of TiO2 NPs on mesenchymal stem cells (hMSCs). Methods: The hMSCs from Wharton`s jelly were exposed to 4, 62.5 and 500 μg/mL of TiO2 NPs. Viability and cell proliferation tests were carried out at 2, 5 and 7 days of exposure. Additionally, the osteogenic and adipogenic differentiation capacity was evaluated. Results: TiO2 NPs had a perinuclear arrangement and internalized in vesicles into cytosol, diminished significantly the viability (40% and 30% and 30%) and the proliferation cellular (35%, 50% and 55%), differentiation to osteoblast (38%) and adipoblasts (20%). Conclusion: TiO2 NPs affect viability, proliferation, and differentiation of hMSCs. Our result suggests more regulation of the use of TiO2.
AB - Background: Titanium dioxide (TiO2) is employed in the production of cosmetics, sunscreen, food, and drugs; however, TiO2 is toxic at the nanometric scale. Objective: To analyze the in vitro toxic effect of TiO2 NPs on mesenchymal stem cells (hMSCs). Methods: The hMSCs from Wharton`s jelly were exposed to 4, 62.5 and 500 μg/mL of TiO2 NPs. Viability and cell proliferation tests were carried out at 2, 5 and 7 days of exposure. Additionally, the osteogenic and adipogenic differentiation capacity was evaluated. Results: TiO2 NPs had a perinuclear arrangement and internalized in vesicles into cytosol, diminished significantly the viability (40% and 30% and 30%) and the proliferation cellular (35%, 50% and 55%), differentiation to osteoblast (38%) and adipoblasts (20%). Conclusion: TiO2 NPs affect viability, proliferation, and differentiation of hMSCs. Our result suggests more regulation of the use of TiO2.
KW - Adipoblasts
KW - Cell differentiation
KW - Mesenchymal stem cell
KW - Nanoparticle
KW - Osteoblasts
KW - Titanium dioxide
KW - Toxicity
KW - Viability
UR - http://www.scopus.com/inward/record.url?scp=85086339565&partnerID=8YFLogxK
U2 - 10.1007/s13273-020-00084-8
DO - 10.1007/s13273-020-00084-8
M3 - Artículo
AN - SCOPUS:85086339565
SN - 1738-642X
VL - 16
SP - 321
EP - 330
JO - Molecular and Cellular Toxicology
JF - Molecular and Cellular Toxicology
IS - 3
ER -
