TY - JOUR
T1 - Tocopherol Accumulation and Temporal Expression Analysis of VTE1 and VTE5 Gene Family in Fruit of Two Contrasting Avocado Genotypes
AU - Valdez-Agramón, Rosalva C.
AU - Valdez-Morales, Maribel
AU - López-Meyer, Melina
AU - Sandoval-Castro, Eduardo
AU - Calderón-Vázquez, Carlos L.
N1 - Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022/6
Y1 - 2022/6
N2 - α-tocopherol is found in high concentrations in avocado fruit mesocarp, however, its accumulation and genetic control during maturation and ripening has not been elucidated. Based in the relevance of VTE1 and VTE5 genes in tocopherol biosynthesis and aiming to determine the association between tocopherol accumulation and expression of tocopherol biosynthetic genes, gene expression of VTE1 and VTE5 were evaluated through the time during three developmental stages: before harvest at 100, 160 and 220 days after flowering (DAF) and after harvest (220 DAF + 5) in two contrasting avocado genotypes (San Miguel and AVO40). San Miguel reached the highest levels at 220 DAF, whereas AVO40 increased α-tocopherol only after ripening (220 DAF + 5). A genome-wide search for VTE1 and VTE5 allowed to identify one and three genes, respectively. Both genotypes showed contrasting patterns of gene expression. Interestingly, AVO40 showed a highly positive correlation between α-tocopherol levels and gene expression of VTE1 and all VTE5 variants. On the other hand, San Miguel showed only a positive correlation between α-tocopherol level and VTE1gene expression.
AB - α-tocopherol is found in high concentrations in avocado fruit mesocarp, however, its accumulation and genetic control during maturation and ripening has not been elucidated. Based in the relevance of VTE1 and VTE5 genes in tocopherol biosynthesis and aiming to determine the association between tocopherol accumulation and expression of tocopherol biosynthetic genes, gene expression of VTE1 and VTE5 were evaluated through the time during three developmental stages: before harvest at 100, 160 and 220 days after flowering (DAF) and after harvest (220 DAF + 5) in two contrasting avocado genotypes (San Miguel and AVO40). San Miguel reached the highest levels at 220 DAF, whereas AVO40 increased α-tocopherol only after ripening (220 DAF + 5). A genome-wide search for VTE1 and VTE5 allowed to identify one and three genes, respectively. Both genotypes showed contrasting patterns of gene expression. Interestingly, AVO40 showed a highly positive correlation between α-tocopherol levels and gene expression of VTE1 and all VTE5 variants. On the other hand, San Miguel showed only a positive correlation between α-tocopherol level and VTE1gene expression.
KW - Avocado
KW - Phytol kinase
KW - Tocopherol cyclase
KW - α-Tocopherol
UR - http://www.scopus.com/inward/record.url?scp=85130737076&partnerID=8YFLogxK
U2 - 10.1007/s11130-022-00977-0
DO - 10.1007/s11130-022-00977-0
M3 - Artículo
C2 - 35618894
AN - SCOPUS:85130737076
SN - 0921-9668
VL - 77
SP - 265
EP - 270
JO - Plant Foods for Human Nutrition
JF - Plant Foods for Human Nutrition
IS - 2
ER -