TY - JOUR
T1 - Time-Course of CYP450 Genes Expression from Dendroctonus rhizophagus (Curculionidae: Scolytinae) during Early Hours of Drilling Bark and Settling into the Host Tree
AU - Sarabia, Laura E.
AU - López, María F.
AU - Pineda-Mendoza, Rosa M.
AU - Obregón-Molina, Gabriel
AU - Gonzalez-Escobedo, Roman
AU - Albores-Medina, Arnulfo
AU - Zúñiga, Gerardo
N1 - Publisher Copyright:
© The Author(s) 2019. Published by Oxford University Press on behalf of Entomological Society of America.
PY - 2019/5/22
Y1 - 2019/5/22
N2 - Dendroctonus bark beetles (Scolytinae) are one of the most important disturbance agents of coniferous forests in North and Central America. These beetles spend their lives almost entirely under the tree bark, and their survival and reproductive success depend on their ability to overcome the toxic effect of the trees' oleoresin. The cytochromes P450 (CYPs) are associated with the detoxification process of xenobiotics, as well as other physiological processes. Different cytochromes (families 4, 6, and 9) in the Dendroctonus species have been expressed under several experimental conditions; nevertheless, the expression time-course of these genes is unknown. To explore the induction speed of CYPs, we evaluated the relative expression of the CYP6BW5, CYP6DG1, CYP6DJ2, CYP9Z18, and CYP9Z20 genes at the early hours of drilling and settling into a tree (1, 2, 4, 6, 8, 12, 18 h) both in females and males, solitary or paired, of the bark beetle Dendroctonus rhizophagus Thomas and Bright. Our findings show that the five genes were rapidly overexpressed in the early hours (1 to 6 h) in both sexes and in solitary and paired conditions, suggesting their participation in the detoxification process. Additionally, the CYPs expression shows up- and down-regulation patterns through these short times, suggesting their probable participation in other physiological processes as the biosynthesis of hormones, pheromones or compounds related to reproduction.
AB - Dendroctonus bark beetles (Scolytinae) are one of the most important disturbance agents of coniferous forests in North and Central America. These beetles spend their lives almost entirely under the tree bark, and their survival and reproductive success depend on their ability to overcome the toxic effect of the trees' oleoresin. The cytochromes P450 (CYPs) are associated with the detoxification process of xenobiotics, as well as other physiological processes. Different cytochromes (families 4, 6, and 9) in the Dendroctonus species have been expressed under several experimental conditions; nevertheless, the expression time-course of these genes is unknown. To explore the induction speed of CYPs, we evaluated the relative expression of the CYP6BW5, CYP6DG1, CYP6DJ2, CYP9Z18, and CYP9Z20 genes at the early hours of drilling and settling into a tree (1, 2, 4, 6, 8, 12, 18 h) both in females and males, solitary or paired, of the bark beetle Dendroctonus rhizophagus Thomas and Bright. Our findings show that the five genes were rapidly overexpressed in the early hours (1 to 6 h) in both sexes and in solitary and paired conditions, suggesting their participation in the detoxification process. Additionally, the CYPs expression shows up- and down-regulation patterns through these short times, suggesting their probable participation in other physiological processes as the biosynthesis of hormones, pheromones or compounds related to reproduction.
KW - CYP450
KW - bark beetle
KW - detoxification
KW - monoterpene
UR - http://www.scopus.com/inward/record.url?scp=85066463908&partnerID=8YFLogxK
U2 - 10.1093/jisesa/iez046
DO - 10.1093/jisesa/iez046
M3 - Artículo
C2 - 31115475
AN - SCOPUS:85066463908
SN - 1536-2442
VL - 19
JO - Journal of Insect Science
JF - Journal of Insect Science
IS - 3
M1 - iez046
ER -