TY - JOUR
T1 - The outer membrane vesicles of aeromonas hydrophilaATCC®7966TM
T2 - A proteomic analysis and effect on host cells
AU - Avila-Calderón, Eric Daniel
AU - Otero-Olarra, Jorge Erick
AU - Flores-Romo, Leopoldo
AU - Peralta, Humberto
AU - Aguilera-Arreola, Ma Guadalupe
AU - Morales-García, María Rosario
AU - Calderón-Amador, Juana
AU - Medina-Chávez, Olin
AU - Donis-Maturano, Luis
AU - Del Socorro Ruiz-Palma, María
AU - Contreras-Rodríguez, Araceli
N1 - Publisher Copyright:
Copyright © 2018 Avila-Calderón, Otero-Olarra, Flores-Romo, Peralta, Aguilera-Arreola, Morales-García, Calderón-Amador, Medina-Chávez, Donis-Maturano, Ruiz-Palma and Contreras-Rodríguez.
PY - 2018/11/16
Y1 - 2018/11/16
N2 - Gram-negative bacteria release outer membrane vesicles (OMVs) into the extracellular environment. OMVs have been studied extensively in bacterial pathogens, however, information related with the composition of Aeromonas hydrophila OMVs is missing. In this study we analyzed the composition of purified OMVs from A. hydrophila ATCC® 7966TM by proteomics. Also we studied the effect of OMVs on human peripheral blood mononuclear cells (PBMCs). Vesicles were grown in agar plates and then purified through ultracentrifugation steps. Purified vesicles showed an average diameter of 90-170 nm. Moreover, 211 unique proteins were found in OMVs from A. hydrophila; some of them are well-known as virulence factors such as: Haemolysin Ahh1, RtxA toxin, extracellular lipase, HcpA protein, among others. OMVs from A. hydrophila ATCC® 7966TM induced lymphocyte activation and apoptosis in monocytes, as well as over-expression of pro-inflammatory cytokines. This work contributed to the knowledge of the composition of the vesicles of A. hydrophila ATCC® 7966TM and their interaction with the host cell.
AB - Gram-negative bacteria release outer membrane vesicles (OMVs) into the extracellular environment. OMVs have been studied extensively in bacterial pathogens, however, information related with the composition of Aeromonas hydrophila OMVs is missing. In this study we analyzed the composition of purified OMVs from A. hydrophila ATCC® 7966TM by proteomics. Also we studied the effect of OMVs on human peripheral blood mononuclear cells (PBMCs). Vesicles were grown in agar plates and then purified through ultracentrifugation steps. Purified vesicles showed an average diameter of 90-170 nm. Moreover, 211 unique proteins were found in OMVs from A. hydrophila; some of them are well-known as virulence factors such as: Haemolysin Ahh1, RtxA toxin, extracellular lipase, HcpA protein, among others. OMVs from A. hydrophila ATCC® 7966TM induced lymphocyte activation and apoptosis in monocytes, as well as over-expression of pro-inflammatory cytokines. This work contributed to the knowledge of the composition of the vesicles of A. hydrophila ATCC® 7966TM and their interaction with the host cell.
KW - Aeromonas
KW - Bacterial vesicles
KW - Gram-negative bacteria
KW - Host cell immune response
KW - Outer membrane vesicles
KW - Pathogens
UR - http://www.scopus.com/inward/record.url?scp=85056879285&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2018.02765
DO - 10.3389/fmicb.2018.02765
M3 - Artículo
C2 - 30519218
SN - 1664-302X
VL - 9
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
IS - NOV
M1 - 2765
ER -