TY - JOUR
T1 - Supramolecular complex formation, a possible antigenotoxic mechanism of glucomannan against aflatoxin B1
AU - Madrigal-Santillán, Eduardo
AU - Madrigal-Bujaidar, Eduardo
AU - Reyes-Arellano, Alicia
AU - Morales-González, José Antonio
AU - Álvarez-González, Isela
AU - Sánchez-Gutiérrez, Manuel
AU - Izquierdo-Vega, Jeannett A.
AU - Calzada-Mendoza, Claudia C.
AU - Anguiano-Robledo, Liliana
AU - Morales-González, Ángel
N1 - Publisher Copyright:
© 2020, © 2020 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2019/9/14
Y1 - 2019/9/14
N2 - Glucomannan is a highly branched polysaccharide with glycosidic linkages, constituted of mannoses and glucoses. In recent years, its usefulness due to its immunological, antioxidant and antimutagenic activity has been recognized. The aim of the study was to determine the antigenotoxic ability of glucomannan extracted from Candida utilis orally administered (100–700 mg/kg) to mice, which subsequently received 1 mg/kg aflatoxin B1. Hepatocytes obtained from these animals 4–16 h post administration were examined by means of the comet assay. The antigenotoxic effect was found to be higher than that observed in previous studies with α-mannan and β-D-glucan isolated from Saccharomyces cerevisiae., In order to explore the possibility of formation of a supramolecular complex between glucomannan and aflatoxin B1, both compounds were co-crystallized, their melting points determined, and the complex analyzed through ultraviolet spectroscopy. The spectroscopy data suggest that the protective effect of glucomannan is related to the formation of a supramolecular complex between the two compounds.
AB - Glucomannan is a highly branched polysaccharide with glycosidic linkages, constituted of mannoses and glucoses. In recent years, its usefulness due to its immunological, antioxidant and antimutagenic activity has been recognized. The aim of the study was to determine the antigenotoxic ability of glucomannan extracted from Candida utilis orally administered (100–700 mg/kg) to mice, which subsequently received 1 mg/kg aflatoxin B1. Hepatocytes obtained from these animals 4–16 h post administration were examined by means of the comet assay. The antigenotoxic effect was found to be higher than that observed in previous studies with α-mannan and β-D-glucan isolated from Saccharomyces cerevisiae., In order to explore the possibility of formation of a supramolecular complex between glucomannan and aflatoxin B1, both compounds were co-crystallized, their melting points determined, and the complex analyzed through ultraviolet spectroscopy. The spectroscopy data suggest that the protective effect of glucomannan is related to the formation of a supramolecular complex between the two compounds.
KW - Aflatoxin B
KW - Candida utilis
KW - comet assay
KW - glucomannan
KW - supramolecular complex
UR - http://www.scopus.com/inward/record.url?scp=85078488269&partnerID=8YFLogxK
U2 - 10.1080/02772248.2020.1715410
DO - 10.1080/02772248.2020.1715410
M3 - Artículo
AN - SCOPUS:85078488269
SN - 0277-2248
VL - 101
SP - 369
EP - 388
JO - Toxicological and Environmental Chemistry
JF - Toxicological and Environmental Chemistry
IS - 7-8
ER -