TY - JOUR
T1 - Striking phenotypic and functional differences in lamina propria lymphocytes from the large and small intestine of mice
AU - Reséndiz-Albor, Aldo Arturo
AU - Esquivel, Rita
AU - López-Revilla, Rubén
AU - Verdín, Leticia
AU - Moreno-Fierros, Leticia
N1 - Funding Information:
This work was supported by CONACyT 34834, 43102 and UNAM DGAPA PAPIIT IN207800 and IN213903 grants. We thank Q.B.P Blanca Estela Reyes Márquez for expert help with flow cytometry and PhD Adriana Jarillo and M. Sc. Miguel Leonardo Martínez for help in several experiments.
PY - 2005/4/29
Y1 - 2005/4/29
N2 - Although intraepithelial T lymphocytes of the large intestine (LI) are known to differ from those of the small intestine (SI) in phenotype and function, differences in LI and SI lamina propria (LP) lymphocyte populations have not been clearly established. In this work we found striking phenotypic differences between SI and LI LP lymphocyte populations from Balb/c mice analyzed by flow cytometry. In the LI most lymphocytes were B cells and the predominant T cells were TCR-αβ+, CD8+. In contrast, in the SI most T lymphocytes were CD4+ expressing TCR-αβ+, although a higher proportion expressed TCR-γδ+ than in the LI. In T cells the expression of adhesion molecules and cytokines was also different between SI and LI. The proportion of LP T cells expressing α4β7 and L-selectin was higher in the LI than in the SI; whereas a greater proportion of cells expressing αEβ7 were detected in the SI than in LI. Higher proportions of T cells expressing L-selectin and α4β1 were detected in the intraepithelial compartment of the LI than that of the SI, whereas the number of T cells expressing αEβ7 was much higher in the SI than in the LI. The proportion of T cells spontaneously producing IL-2, IFNγ, and IL-4 at the intraepithelial and lamina propria, in the small and large intestine, was different indicating that distinctive functional features exist in the lymphocyte populations residing at the different intestinal compartments.
AB - Although intraepithelial T lymphocytes of the large intestine (LI) are known to differ from those of the small intestine (SI) in phenotype and function, differences in LI and SI lamina propria (LP) lymphocyte populations have not been clearly established. In this work we found striking phenotypic differences between SI and LI LP lymphocyte populations from Balb/c mice analyzed by flow cytometry. In the LI most lymphocytes were B cells and the predominant T cells were TCR-αβ+, CD8+. In contrast, in the SI most T lymphocytes were CD4+ expressing TCR-αβ+, although a higher proportion expressed TCR-γδ+ than in the LI. In T cells the expression of adhesion molecules and cytokines was also different between SI and LI. The proportion of LP T cells expressing α4β7 and L-selectin was higher in the LI than in the SI; whereas a greater proportion of cells expressing αEβ7 were detected in the SI than in LI. Higher proportions of T cells expressing L-selectin and α4β1 were detected in the intraepithelial compartment of the LI than that of the SI, whereas the number of T cells expressing αEβ7 was much higher in the SI than in the LI. The proportion of T cells spontaneously producing IL-2, IFNγ, and IL-4 at the intraepithelial and lamina propria, in the small and large intestine, was different indicating that distinctive functional features exist in the lymphocyte populations residing at the different intestinal compartments.
KW - Intestinal lymphocyte isolation
KW - Intestinal regionalization
KW - Lamina propria lymphocytes
KW - Large intestine
UR - http://www.scopus.com/inward/record.url?scp=16244423347&partnerID=8YFLogxK
U2 - 10.1016/j.lfs.2004.08.042
DO - 10.1016/j.lfs.2004.08.042
M3 - Artículo
C2 - 15808880
SN - 0024-3205
VL - 76
SP - 2783
EP - 2803
JO - Life Sciences
JF - Life Sciences
IS - 24
ER -