TY - JOUR
T1 - Sperm quality in relation to age and weight of white shrimp Litopenaeus vannamei
AU - Ceballos-Vázquez, B. P.
AU - Rosas, C.
AU - Racotta, I. S.
N1 - Funding Information:
This research was supported by the following projects: CIBNOR-PAC15, SIMAC 00BCS7501, INFOTEC-CONACYT 100-2 and CGPI 2002037. Thanks to Cristina Pascual for her advice in the techniques of sperm quality determination. The editorial staff at CIBNOR provides editing of the English text. B.P. Ceballos-Vázquez is a PhD student-fellow of Consejo Nacional de Ciencia y Tecnologı́a, and the results presented here are part of her PhD thesis. She also receives fellowships from Instituto Politécnico Nacional (Estı́mulo al Desempeño en Investigación, and Comisión de Operación y Fomento de Actividades Académicas).
PY - 2003/12/1
Y1 - 2003/12/1
N2 - Optimal sperm quality is an important feature for management of shrimp broodstock. The understanding of the sperm quality in relation to age and weight of shrimp is useful to select male broodstock. To evaluate the effect of age and weight on sperm quality, Litopenaeus vannamei males from the same cohort at ages of 6, 8, 10, and 12 months were examined, using body weight as a covariable in ANCOVA. Additionally, males of the same cohort at an age of 12 months, but from different culture conditions, were compared. Spermatophore weight, sperm count, and percentage of normal sperm were positively correlated to body weight. Sperm count was positively correlated to spermatophore weight and percentage of normal sperm. Males at an age of 12 months had superior sperm quality than younger males, based on observations of larger spermatophore weight, higher sperm count, and higher percentage of normal sperm. By eliminating the influence of body weight with the use of ANCOVA, the same effect was still observed, indicating that the effect of age of males on sperm quality was independent of body weight. However, when large differences in growth were forced by changing culture conditions, sperm quality differences were observed and could be explained by body weight. In conclusion, using 12-month-old males would lead to improved shrimp seed production, since they are mature males with high sperm quality. In addition, for improved productivity, it is important to consider the culture conditions under which domesticated male broodstock will be obtained.
AB - Optimal sperm quality is an important feature for management of shrimp broodstock. The understanding of the sperm quality in relation to age and weight of shrimp is useful to select male broodstock. To evaluate the effect of age and weight on sperm quality, Litopenaeus vannamei males from the same cohort at ages of 6, 8, 10, and 12 months were examined, using body weight as a covariable in ANCOVA. Additionally, males of the same cohort at an age of 12 months, but from different culture conditions, were compared. Spermatophore weight, sperm count, and percentage of normal sperm were positively correlated to body weight. Sperm count was positively correlated to spermatophore weight and percentage of normal sperm. Males at an age of 12 months had superior sperm quality than younger males, based on observations of larger spermatophore weight, higher sperm count, and higher percentage of normal sperm. By eliminating the influence of body weight with the use of ANCOVA, the same effect was still observed, indicating that the effect of age of males on sperm quality was independent of body weight. However, when large differences in growth were forced by changing culture conditions, sperm quality differences were observed and could be explained by body weight. In conclusion, using 12-month-old males would lead to improved shrimp seed production, since they are mature males with high sperm quality. In addition, for improved productivity, it is important to consider the culture conditions under which domesticated male broodstock will be obtained.
KW - Normal sperm
KW - Penaeus
KW - Reproduction
KW - Sperm count
UR - http://www.scopus.com/inward/record.url?scp=0345393070&partnerID=8YFLogxK
U2 - 10.1016/S0044-8486(03)00322-3
DO - 10.1016/S0044-8486(03)00322-3
M3 - Artículo
SN - 0044-8486
VL - 228
SP - 141
EP - 151
JO - Aquaculture
JF - Aquaculture
IS - 1-4
ER -