TY - JOUR
T1 - Solid culture amended with small amounts of raw coffee beans for the removal of petroleum hydrocarbon from weathered contaminated soil
AU - Roldán-Martín, Adriana
AU - Calva-Calva, Graciano
AU - Rojas-Avelizapa, Norma
AU - Díaz-Cervantes, Ma Dolores
AU - Rodríguez-Vázquez, Refugio
N1 - Funding Information:
This work was supported by the Mexico National Council for Science and Technology (CONACYT) and the Mexican Institute of Petroleum (IMP), Grant 2002-06-VI. Thanks also are due to Ing. Elvira García, the Mexican Coffee Council, and Alfredo Medina for their technical assistance.
PY - 2007/7
Y1 - 2007/7
N2 - Solid culture with small amounts of low-quality raw coffee beans was used for total petroleum hydrocarbon (TPH) removal from a weathered and polluted soil. Soil contaminated with 58 000 mg kg-1 of TPH was treated with soil:coffee bean ratios of 98:2, 96:4, 94:6, and 92:8, at a C:N:P ratio of 100:10:1, 20% humidity, and 28 °C, for periods of 15, 60, and 90 days. The highest TPH removal (63%) was obtained with a soil/bean ratio of 98:2 over 15 days, corresponding with the highest rates of microbial respiration and the greatest increases in bacterial and fungal counts to 9 lnCFU and 6 lnCFU, respectively. Scanning electron microscopy showed high fungal colonization of coffee beans, with Mucor sp., Aspergillus sp., Aspergillus niger., and Penicillium sp. growing on TPH as sole carbon source.
AB - Solid culture with small amounts of low-quality raw coffee beans was used for total petroleum hydrocarbon (TPH) removal from a weathered and polluted soil. Soil contaminated with 58 000 mg kg-1 of TPH was treated with soil:coffee bean ratios of 98:2, 96:4, 94:6, and 92:8, at a C:N:P ratio of 100:10:1, 20% humidity, and 28 °C, for periods of 15, 60, and 90 days. The highest TPH removal (63%) was obtained with a soil/bean ratio of 98:2 over 15 days, corresponding with the highest rates of microbial respiration and the greatest increases in bacterial and fungal counts to 9 lnCFU and 6 lnCFU, respectively. Scanning electron microscopy showed high fungal colonization of coffee beans, with Mucor sp., Aspergillus sp., Aspergillus niger., and Penicillium sp. growing on TPH as sole carbon source.
KW - Bioremediation
KW - Coffee bean
KW - Nutrients
KW - Solid culture
KW - Total petroleum hydrocarbons
UR - http://www.scopus.com/inward/record.url?scp=34249008131&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2006.10.008
DO - 10.1016/j.ibiod.2006.10.008
M3 - Artículo
SN - 0964-8305
VL - 60
SP - 35
EP - 39
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
IS - 1
ER -