TY - JOUR
T1 - Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production
AU - Pérez-Pimienta, Jose A.
AU - Vargas-Tah, Alejandra
AU - López-Ortega, Karla M.
AU - Medina-López, Yessenia N.
AU - Mendoza-Pérez, Jorge A.
AU - Avila, Sayeny
AU - Singh, Seema
AU - Simmons, Blake A.
AU - Loaces, Inés
AU - Martinez, Alfredo
N1 - Publisher Copyright:
© 2016 Elsevier Ltd
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Agave bagasse (AGB) has gained recognition as a drought-tolerant biofuel feedstock with high productivity in semiarid regions. A comparative analysis of ionic liquid (IL) and organosolv (OV) pretreatment technologies in AGB was performed using a sequential enzymatic saccharification and fermentation (SESF) strategy with cellulolytic enzymes and the ethanologenic Escherichia coli strain MS04. After pretreatment, 86% of xylan and 45% of lignin were removed from OV-AGB, whereas IL-AGB reduced lignin content by 28% and xylan by 50% when compared to the untreated biomass. High glucan (>90%) and xylan (>83%) conversion was obtained with both pretreated samples. During the fermentation stage (48 h), 12.1 and 12.7 kg of ethanol were produced per 100 kg of untreated AGB for IL and OV, respectively. These comparative analyses showed the advantages of SESF using IL and OV in a biorefinery configuration where a better understanding of AGB recalcitrance is key for future applications.
AB - Agave bagasse (AGB) has gained recognition as a drought-tolerant biofuel feedstock with high productivity in semiarid regions. A comparative analysis of ionic liquid (IL) and organosolv (OV) pretreatment technologies in AGB was performed using a sequential enzymatic saccharification and fermentation (SESF) strategy with cellulolytic enzymes and the ethanologenic Escherichia coli strain MS04. After pretreatment, 86% of xylan and 45% of lignin were removed from OV-AGB, whereas IL-AGB reduced lignin content by 28% and xylan by 50% when compared to the untreated biomass. High glucan (>90%) and xylan (>83%) conversion was obtained with both pretreated samples. During the fermentation stage (48 h), 12.1 and 12.7 kg of ethanol were produced per 100 kg of untreated AGB for IL and OV, respectively. These comparative analyses showed the advantages of SESF using IL and OV in a biorefinery configuration where a better understanding of AGB recalcitrance is key for future applications.
KW - Agave bagasse
KW - Crystallinity
KW - Fuel ethanol
KW - Ionic liquid
KW - Organosolv
KW - Sequential saccharification and fermentation
UR - http://www.scopus.com/inward/record.url?scp=84997079766&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2016.11.064
DO - 10.1016/j.biortech.2016.11.064
M3 - Artículo
C2 - 27889478
SN - 0960-8524
VL - 225
SP - 191
EP - 198
JO - Bioresource Technology
JF - Bioresource Technology
ER -