TY - JOUR
T1 - Selective iNOS inhibition reduces renal damage induced by cisplatin
AU - Chirino, Yolanda I.
AU - Trujillo, Joyce
AU - Sánchez-González, Dolores Javier
AU - Martínez-Martínez, Claudia María
AU - Cruz, Cristino
AU - Bobadilla, Norma A.
AU - Pedraza-Chaverri, José
N1 - Funding Information:
This work was supported by UNAM (DGAPA IN227103 and DGAPA IN228206-3).
PY - 2008/1/4
Y1 - 2008/1/4
N2 - Cisplatin is a chemotherapeutic agent used in the treatment of several cancer tumors; however, nephrotoxicity has restricted its use. Reactive oxygen species and peroxynitrite, which is formed by the reaction between superoxide anion and nitric oxide (NO{radical dot}), are implicated in cisplatin-induced nephrotoxicity. In contrast, both toxic and beneficial effects of NO{radical dot} have been suggested in cisplatin-induced nephrotoxicity. Therefore, nowadays the role of NO{radical dot} in this experimental model remains controversial. The aim of the present work was to elucidate the role of NO{radical dot} in cisplatin-induced renal damage using N-[3-(aminomethyl)benzyl]acetamidine (1400W), a selective and irreversible inhibitor of iNOS. The mRNA levels of iNOS were increased in cisplatin-treated rats. The administration of 1400W reduced the cisplatin induced histological damage, renal dysfunction (increase in proteinuria and kidney injury molecule expression and decrease in creatinine clearance), tubulointerstitial infiltration, oxidative stress (increase in renal malondialdehyde and inmmunostaining for 4-hydroxy-2-nonenal) and nitrosative stress (immunostaining for 3-nitrotyrosine). In addition, the administration of 1400W was unable to modify systolic blood pressure in control rats. Our data demonstrate that selective iNOS inhibition reduces the cisplatin-induced nephrotoxicity and nitrosative stress which strongly suggest that in this experimental model (1) the NO{radical dot} production is toxic and (2) iNOS is the main source of NO{radical dot}.
AB - Cisplatin is a chemotherapeutic agent used in the treatment of several cancer tumors; however, nephrotoxicity has restricted its use. Reactive oxygen species and peroxynitrite, which is formed by the reaction between superoxide anion and nitric oxide (NO{radical dot}), are implicated in cisplatin-induced nephrotoxicity. In contrast, both toxic and beneficial effects of NO{radical dot} have been suggested in cisplatin-induced nephrotoxicity. Therefore, nowadays the role of NO{radical dot} in this experimental model remains controversial. The aim of the present work was to elucidate the role of NO{radical dot} in cisplatin-induced renal damage using N-[3-(aminomethyl)benzyl]acetamidine (1400W), a selective and irreversible inhibitor of iNOS. The mRNA levels of iNOS were increased in cisplatin-treated rats. The administration of 1400W reduced the cisplatin induced histological damage, renal dysfunction (increase in proteinuria and kidney injury molecule expression and decrease in creatinine clearance), tubulointerstitial infiltration, oxidative stress (increase in renal malondialdehyde and inmmunostaining for 4-hydroxy-2-nonenal) and nitrosative stress (immunostaining for 3-nitrotyrosine). In addition, the administration of 1400W was unable to modify systolic blood pressure in control rats. Our data demonstrate that selective iNOS inhibition reduces the cisplatin-induced nephrotoxicity and nitrosative stress which strongly suggest that in this experimental model (1) the NO{radical dot} production is toxic and (2) iNOS is the main source of NO{radical dot}.
KW - 1400W
KW - Cisplatin
KW - Inducible nitric oxide synthase (iNOS)
KW - Nitric oxide (NO{radical dot})
UR - http://www.scopus.com/inward/record.url?scp=37049025204&partnerID=8YFLogxK
U2 - 10.1016/j.toxlet.2007.10.006
DO - 10.1016/j.toxlet.2007.10.006
M3 - Artículo
C2 - 18063323
SN - 0378-4274
VL - 176
SP - 48
EP - 57
JO - Toxicology Letters
JF - Toxicology Letters
IS - 1
ER -