TY - JOUR
T1 - Ripening in papaya fruit is altered by ACC oxidase cosuppression
AU - López-Gómez, Rodolfo
AU - Cabrera-Ponce, Jose Luis
AU - Saucedo-Arias, Luis Jorge
AU - Carreto-Montoya, Lorena
AU - Villanueva-Arce, Ramon
AU - Díaz-Perez, Juan Carlos
AU - Gómez-Lim, Miguel Angel
AU - Herrera-Estrella, Luis
PY - 2009/2
Y1 - 2009/2
N2 - Papaya (Carica papaya) is a very important crop in many tropical countries but it is highly susceptible to parasitic diseases, physiological disorders, mechanical damage and fruit overripening. Here we report a study on ACC oxidase cosuppression and its effects on papaya fruit ripening. Papaya ACC oxidase was isolated using PCR and embriogenic cells transformed by biolistic using the CaMV 35S promoter to drive the expression of the PCR fragment in sense orientation. Fifty transgenic lines were recovered and 20 of those were grown under field conditions. Southern analysis showed incorporation of the transgene in different copy numbers in the papaya genome. Fruits were evaluated in terms of texture (firmness), colour development, respiration and ethylene production. A sharp reduction in ethylene and CO2 production was detected, whereas softening and colour development of the peel were also altered. Overall, transgenic fruits showed a delay in ripening rate. A reduction in mRNA level for ACC oxidase in transgenic fruit was clearly detectable by northern blot. More studies are necessary before this technology can be used to extend the shelf life of papaya fruit.
AB - Papaya (Carica papaya) is a very important crop in many tropical countries but it is highly susceptible to parasitic diseases, physiological disorders, mechanical damage and fruit overripening. Here we report a study on ACC oxidase cosuppression and its effects on papaya fruit ripening. Papaya ACC oxidase was isolated using PCR and embriogenic cells transformed by biolistic using the CaMV 35S promoter to drive the expression of the PCR fragment in sense orientation. Fifty transgenic lines were recovered and 20 of those were grown under field conditions. Southern analysis showed incorporation of the transgene in different copy numbers in the papaya genome. Fruits were evaluated in terms of texture (firmness), colour development, respiration and ethylene production. A sharp reduction in ethylene and CO2 production was detected, whereas softening and colour development of the peel were also altered. Overall, transgenic fruits showed a delay in ripening rate. A reduction in mRNA level for ACC oxidase in transgenic fruit was clearly detectable by northern blot. More studies are necessary before this technology can be used to extend the shelf life of papaya fruit.
KW - ACC oxidase
KW - Biolistic
KW - Climacteric
KW - Cosuppression
KW - Ethylene
KW - Fruit ripening
KW - Papaya
UR - http://www.scopus.com/inward/record.url?scp=58349091878&partnerID=8YFLogxK
U2 - 10.1007/s11248-008-9197-0
DO - 10.1007/s11248-008-9197-0
M3 - Artículo
SN - 0962-8819
VL - 18
SP - 89
EP - 97
JO - Transgenic Research
JF - Transgenic Research
IS - 1
ER -