TY - JOUR
T1 - Rich fatty acids diet of fish and olive oils modifies membrane properties in striatal rat synaptosomes
AU - Morales-Martínez, Adriana
AU - Zamorano-Carrillo, Absalom
AU - Montes, Sergio
AU - El-Hafidi, Mohammed
AU - Sánchez-Mendoza, Alicia
AU - Soria-Castro, Elizabeth
AU - Martínez-Lazcano, Juan Carlos
AU - Martínez-Gopar, Pablo Eliasib
AU - Ríos, Camilo
AU - Pérez-Severiano, Francisca
N1 - Publisher Copyright:
© 2019 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2021
Y1 - 2021
N2 - Background: Essential fatty acids (EFAs) and non-essential fatty acids (nEFAs) exert experimental and clinical neuroprotection in neurodegenerative diseases. The main EFAs, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), nEFAs, and oleic acid (OA) contained in olive and fish oils are inserted into the cell membranes, but the exact mechanism through which they exert neuroprotection is still unknown. Objectives and Methods: In this study, we assessed the fatty acids content and membrane fluidity in striatal rat synaptosomes after fatty acid-rich diets (olive- or a fish-oil diet, 15% w/w). Then, we evaluated the effect of enriching striatum synaptosomes with fatty acids on the oxidative damage produced by the prooxidants ferrous sulfate (FeSO4) or quinolinic acid (QUIN). Results and Discussion: Lipid profile analysis in striatal synaptosomes showed that EPA content increased in the fish oil group in comparison with control and olive groups. Furthermore, we found that synaptosomes enriched with fatty acids and incubated with QUIN or FeSO4 showed a significant oxidative damage reduction. Results suggest that EFAs, particularly EPA, improve membrane fluidity and confer antioxidant effect.
AB - Background: Essential fatty acids (EFAs) and non-essential fatty acids (nEFAs) exert experimental and clinical neuroprotection in neurodegenerative diseases. The main EFAs, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), nEFAs, and oleic acid (OA) contained in olive and fish oils are inserted into the cell membranes, but the exact mechanism through which they exert neuroprotection is still unknown. Objectives and Methods: In this study, we assessed the fatty acids content and membrane fluidity in striatal rat synaptosomes after fatty acid-rich diets (olive- or a fish-oil diet, 15% w/w). Then, we evaluated the effect of enriching striatum synaptosomes with fatty acids on the oxidative damage produced by the prooxidants ferrous sulfate (FeSO4) or quinolinic acid (QUIN). Results and Discussion: Lipid profile analysis in striatal synaptosomes showed that EPA content increased in the fish oil group in comparison with control and olive groups. Furthermore, we found that synaptosomes enriched with fatty acids and incubated with QUIN or FeSO4 showed a significant oxidative damage reduction. Results suggest that EFAs, particularly EPA, improve membrane fluidity and confer antioxidant effect.
KW - Membrane fluidity
KW - antioxidants
KW - fatty acids
KW - fish oil
KW - olive oil
KW - oxidative stress
KW - striatum
KW - synaptosomes
UR - http://www.scopus.com/inward/record.url?scp=85062464983&partnerID=8YFLogxK
U2 - 10.1080/1028415X.2019.1584692
DO - 10.1080/1028415X.2019.1584692
M3 - Artículo
C2 - 30822260
SN - 1028-415X
VL - 24
SP - 1
EP - 12
JO - Nutritional Neuroscience
JF - Nutritional Neuroscience
IS - 1
ER -