TY - JOUR
T1 - Revealing the 1H NMR metabolome of mirasol chili peppers (Capsicum annuum) infected by Candidatus Phytoplasma trifolii
AU - Velásquez-Valle, Rodolfo
AU - Villa-Ruano, Nemesio
AU - Hidalgo-Martínez, Diego
AU - Zepeda-Vallejo, L. Gerardo
AU - Pérez-Hernández, Nury
AU - Reyes-López, Cesar A.
AU - Reyes-Cervantes, Eric
AU - Medina-Melchor, Diana L.
AU - Becerra-Martínez, Elvia
N1 - Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2020/5
Y1 - 2020/5
N2 - The infection of Capsicum annuum cv. mirasol by Candidatus Phytoplasma trifolii (16SrVI) causes devastating crop losses in northern Mexico. This study addresses the metabolomics profiling of mirasol chili peppers (Capsicum annuum cv. mirasol) infected by Candidatus Phytoplasma trifolii. For this study, 25 diseased fruits and 25 healthy fruits were used. Principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) revealed dramatic changes in the content of 42 metabolites which were identified in diseased and healthy mirasol chili peppers. The endogenous levels of fructose, glucose and formic acid were substantially decreased in the diseased chili peppers. In the same group of samples, high concentrations of alanine, asparagine, fumaric acid, sucrose and threonine were observed. The content of Choline didńt present a significant difference. This evidence supports the fact that Candidatus Phytoplasma trifolii infection reduces de CO2 fixation into carbohydrates, decreases invertase activity, and inhibits glycolysis in the diseased plant tissues. The levels of ascorbic acid, capsaicin and dihydrocapsaicinin in diseased fruits were dramatically decreased, suggesting that Candidatus Phytoplasma trifolii can reduce the pungency and the nutraceutical value of mirasol chili peppers.
AB - The infection of Capsicum annuum cv. mirasol by Candidatus Phytoplasma trifolii (16SrVI) causes devastating crop losses in northern Mexico. This study addresses the metabolomics profiling of mirasol chili peppers (Capsicum annuum cv. mirasol) infected by Candidatus Phytoplasma trifolii. For this study, 25 diseased fruits and 25 healthy fruits were used. Principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) revealed dramatic changes in the content of 42 metabolites which were identified in diseased and healthy mirasol chili peppers. The endogenous levels of fructose, glucose and formic acid were substantially decreased in the diseased chili peppers. In the same group of samples, high concentrations of alanine, asparagine, fumaric acid, sucrose and threonine were observed. The content of Choline didńt present a significant difference. This evidence supports the fact that Candidatus Phytoplasma trifolii infection reduces de CO2 fixation into carbohydrates, decreases invertase activity, and inhibits glycolysis in the diseased plant tissues. The levels of ascorbic acid, capsaicin and dihydrocapsaicinin in diseased fruits were dramatically decreased, suggesting that Candidatus Phytoplasma trifolii can reduce the pungency and the nutraceutical value of mirasol chili peppers.
KW - Candidatus Phytoplasma trifolii
KW - Capsicum annuum cv. mirasol
KW - Metabolomic fingerprinting
KW - Multivariate analysis
KW - Nuclear magnetic resonance spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85078487804&partnerID=8YFLogxK
U2 - 10.1016/j.foodres.2019.108863
DO - 10.1016/j.foodres.2019.108863
M3 - Artículo
C2 - 32247466
AN - SCOPUS:85078487804
SN - 0963-9969
VL - 131
JO - Food Research International
JF - Food Research International
M1 - 108863
ER -