TY - JOUR
T1 - Response of ustilago maydis against the stress caused by three polycationic chitin derivatives
AU - Olicón-Hernández, Dario Rafael
AU - Uribe-Alvarez, Cristina
AU - Uribe-Carvajal, Salvador
AU - Pardo, Juan Pablo
AU - Guerra-Sánchez, Guadalupe
N1 - Publisher Copyright:
© 2017 by the authors.
PY - 2017/12
Y1 - 2017/12
N2 - Chitosan is a stressing molecule that affects the cells walls and plasma membrane of fungi. For chitosan derivatives, the action mode is not clear. In this work, we used the yeast Ustilago maydis to study the effects of these molecules on the plasma membrane, focusing on physiologic and stress responses to chitosan (CH), oligochitosan (OCH), and glycol-chitosan (GCH). Yeasts were cultured with each of these molecules at 1 mg•mL-1 in minimal medium. To compare plasma membrane damage, cells were cultivated in isosmolar medium. Membrane potential (D) as well as oxidative stress were measured. Changes in the total plasma membrane phospholipid and protein profiles were analyzed using standard methods, and fluorescence-stained mitochondria were observed. High osmolarity did not protect against CH inhibition and neither affected membrane potential. The OCH did produce higher oxidative stress. The effects of these molecules were evidenced by modifications in the plasma membrane protein profile. Also, mitochondrial damage was evident for CH and OCH, while GCH resulted in thicker cells with fewer mitochondria and higher glycogen accumulation.
AB - Chitosan is a stressing molecule that affects the cells walls and plasma membrane of fungi. For chitosan derivatives, the action mode is not clear. In this work, we used the yeast Ustilago maydis to study the effects of these molecules on the plasma membrane, focusing on physiologic and stress responses to chitosan (CH), oligochitosan (OCH), and glycol-chitosan (GCH). Yeasts were cultured with each of these molecules at 1 mg•mL-1 in minimal medium. To compare plasma membrane damage, cells were cultivated in isosmolar medium. Membrane potential (D) as well as oxidative stress were measured. Changes in the total plasma membrane phospholipid and protein profiles were analyzed using standard methods, and fluorescence-stained mitochondria were observed. High osmolarity did not protect against CH inhibition and neither affected membrane potential. The OCH did produce higher oxidative stress. The effects of these molecules were evidenced by modifications in the plasma membrane protein profile. Also, mitochondrial damage was evident for CH and OCH, while GCH resulted in thicker cells with fewer mitochondria and higher glycogen accumulation.
KW - Chitosan
KW - Glycol-chitosan
KW - Oligochitosan
KW - Stress response
KW - Ustilago maydis
UR - http://www.scopus.com/inward/record.url?scp=85040227630&partnerID=8YFLogxK
U2 - 10.3390/molecules22121745
DO - 10.3390/molecules22121745
M3 - Artículo
C2 - 29215563
SN - 1420-3049
VL - 22
JO - Molecules
JF - Molecules
IS - 12
M1 - 1745
ER -