TY - JOUR
T1 - Reporting detection of Chlamydia trachomatis DNA in tissues of neonatal death cases
AU - Hernandez-Trejo, Maria
AU - Herrera-Gonzalez, Norma E.
AU - Escobedo-Guerra, Marcos R.
AU - De Haro-Cruz, M. De Jesus
AU - Moreno-Verduzco, Elsa R.
AU - Lopez-Hurtado, Marcela
AU - Guerra-Infante, Fernando M.
PY - 2014/3
Y1 - 2014/3
N2 - Objective to determine whether C. trachomatis was present in neonates with infection, but without an isolated pathogen, who died during the first week of life. Methods early neonatal death cases whose causes of death had been previously adjudicated by the institutional mortality committee were randomly selected. End-point and real-time polymerase chain reaction of the C. trachomatis omp1 gene was used to blindly identify the presence of chlamydial DNA in the paraffinized samples of five organs (from authorized autopsies) of each of the dead neonates. Additionally, differential diagnoses were conducted by amplifying a fragment of the 16S rRNA of Mycoplasma spp. Results in five cases (35.7%), C. trachomatis DNA was found in one or more organs. Severe neonatal infection was present in three cases; one of them corresponded to genotype D of C. trachomatis. Interestingly, another case fulfilled the same criteria but had a positive polymerase chain reaction for Mycoplasma hominis, a pathogen known to produce sepsis in newborns. Conclusion the use of molecular biology techniques in these cases of early infant mortality demonstrated that C. trachomatis could play a role in the development of severe infection and in early neonatal death, similarly to that observed with Mycoplasma hominis. Further study is required to determine the pathogenesis of this perinatal infection.
AB - Objective to determine whether C. trachomatis was present in neonates with infection, but without an isolated pathogen, who died during the first week of life. Methods early neonatal death cases whose causes of death had been previously adjudicated by the institutional mortality committee were randomly selected. End-point and real-time polymerase chain reaction of the C. trachomatis omp1 gene was used to blindly identify the presence of chlamydial DNA in the paraffinized samples of five organs (from authorized autopsies) of each of the dead neonates. Additionally, differential diagnoses were conducted by amplifying a fragment of the 16S rRNA of Mycoplasma spp. Results in five cases (35.7%), C. trachomatis DNA was found in one or more organs. Severe neonatal infection was present in three cases; one of them corresponded to genotype D of C. trachomatis. Interestingly, another case fulfilled the same criteria but had a positive polymerase chain reaction for Mycoplasma hominis, a pathogen known to produce sepsis in newborns. Conclusion the use of molecular biology techniques in these cases of early infant mortality demonstrated that C. trachomatis could play a role in the development of severe infection and in early neonatal death, similarly to that observed with Mycoplasma hominis. Further study is required to determine the pathogenesis of this perinatal infection.
KW - Chlamydia trachomatis
KW - Mortality
KW - Newborn
KW - Polymerase chain reaction
KW - Sepsis
UR - http://www.scopus.com/inward/record.url?scp=84896736121&partnerID=8YFLogxK
U2 - 10.1016/j.jped.2013.09.002
DO - 10.1016/j.jped.2013.09.002
M3 - Artículo
C2 - 24184305
SN - 0021-7557
VL - 90
SP - 182
EP - 189
JO - Jornal de Pediatria
JF - Jornal de Pediatria
IS - 2
ER -