TY - JOUR
T1 - Re-identification of Aeromonas isolates from rainbow trout and incidence of class 1 integron and β-lactamase genes
AU - Vega-Sánchez, Vicente
AU - Latif-Eugenín, Fadua
AU - Soriano-Vargas, Edgardo
AU - Beaz-Hidalgo, Roxana
AU - Figueras, María José
AU - Aguilera-Arreola, Ma Guadalupe
AU - Castro-Escarpulli, Graciela
N1 - Funding Information:
This study was financially supported by Consejo Nacional de Ciencia y Tecnología (CONACYT) , project CB-2008-01-103142 ( UAEM1900/2010C ) and grants 20120652, 20130601 and 20140854 from SIP-IPN , México City. Ma. Guadalupe Aguilera-Arreola and Graciela Castro-Escarpulli received COFAA, EDI and SNI support, Vicente Vega-Sánchez held a scholarship from CONACYT. This work was also supported in part by the project with reference AGL2011-30461-C02-02 by the Ministerio de Ciencia e Innovación (Spain).
PY - 2014/8/27
Y1 - 2014/8/27
N2 - Forty-eight Aeromonas isolates from rainbow trout previously identified by the 16S rDNA-RFLP technique were re-identified using 2 housekeeping genes (gyrB and rpoD). After sequencing the prevalences of the species were A. veronii (29.2%), A. bestiarum (20.8%), A. hydrophila (16.7%), A. sobria (10.4%), A. media (8.3%), A. popoffii (6.2%), A. allosaccharophila (2.1%), A. caviae (2.1%), A. salmonicida (2.1%) and one isolate (2.1%) belongs to a candidate new species "Aeromonas lusitana". Coincident identification results to the 16S rDNA-RFLP technique were only obtained for 68.8% of the isolates. PCR amplification of the enterobacterial repetitive intergenic consensus (ERIC-PCR) indicated that the 48 isolates belonged to 33 different ERIC genotypes. Several genotypes were isolated from different farms and organs in the same fish, indicating a systemic dissemination of the bacteria. The presence of genes (blaIMP, blaCphA/IMIS, blaTEM, blaSHV and intI1) that encode extended-spectrum beta-lactamases (ESBLs), metallo-beta-lactamases (MBLs) and class 1 integrons were studied by PCR. Only 39.6% (19/48) of the strains showed the presence of one or more resistance genes. The gene blaCphA/IMIS was detected in 29.2% of the isolates, followed by the intI1 (6.2%) and blaSHV (4.2%) genes. The variable region of class 1 integrons of the 3 positive isolates was sequenced revealing the presence of the gene cassette aadA1 (aminoglycoside transferase) that plays a role in streptomycin/spectinomycin resistance.
AB - Forty-eight Aeromonas isolates from rainbow trout previously identified by the 16S rDNA-RFLP technique were re-identified using 2 housekeeping genes (gyrB and rpoD). After sequencing the prevalences of the species were A. veronii (29.2%), A. bestiarum (20.8%), A. hydrophila (16.7%), A. sobria (10.4%), A. media (8.3%), A. popoffii (6.2%), A. allosaccharophila (2.1%), A. caviae (2.1%), A. salmonicida (2.1%) and one isolate (2.1%) belongs to a candidate new species "Aeromonas lusitana". Coincident identification results to the 16S rDNA-RFLP technique were only obtained for 68.8% of the isolates. PCR amplification of the enterobacterial repetitive intergenic consensus (ERIC-PCR) indicated that the 48 isolates belonged to 33 different ERIC genotypes. Several genotypes were isolated from different farms and organs in the same fish, indicating a systemic dissemination of the bacteria. The presence of genes (blaIMP, blaCphA/IMIS, blaTEM, blaSHV and intI1) that encode extended-spectrum beta-lactamases (ESBLs), metallo-beta-lactamases (MBLs) and class 1 integrons were studied by PCR. Only 39.6% (19/48) of the strains showed the presence of one or more resistance genes. The gene blaCphA/IMIS was detected in 29.2% of the isolates, followed by the intI1 (6.2%) and blaSHV (4.2%) genes. The variable region of class 1 integrons of the 3 positive isolates was sequenced revealing the presence of the gene cassette aadA1 (aminoglycoside transferase) that plays a role in streptomycin/spectinomycin resistance.
KW - Aeromonas
KW - ESBLs
KW - GyrB
KW - Integrons
KW - Rainbow trout
KW - RpoD
UR - http://www.scopus.com/inward/record.url?scp=84908620107&partnerID=8YFLogxK
U2 - 10.1016/j.vetmic.2014.06.012
DO - 10.1016/j.vetmic.2014.06.012
M3 - Artículo
SN - 0378-1135
VL - 172
SP - 528
EP - 533
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 3-4
ER -