TY - JOUR
T1 - Rapamycin induces morphological and physiological changes without increase in lipid content in Ustilago maydis
AU - Romero-Aguilar, Lucero
AU - Guerra-Sánchez, Guadalupe
AU - Tenorio, Eda P.
AU - Tapia-Rodriguez, Miguel
AU - Matus-Ortega, Genaro
AU - Flores-Herrera, Oscar
AU - González, James
AU - Pardo, Juan Pablo
N1 - Publisher Copyright:
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2020/7/1
Y1 - 2020/7/1
N2 - The evolutionarily conserved serine/threonine kinase TOR recruits different subunits to assemble the Target of Rapamycin Complex 1 (TORC1), which is inhibited by rapamycin and regulates ribosome biogenesis, autophagy, and lipid metabolism by regulating the expression of lipogenic genes. In addition, TORC1 participates in the cell cycle, increasing the length of the G2 phase. In the present work, we investigated the effect of rapamycin on cell growth, cell morphology and neutral lipid metabolism in the phytopathogenic fungus Ustilago maydis. Inhibition of TORC1 by rapamycin induced the formation of septa that separate the nuclei that were formed after mitosis. Regarding neutral lipid metabolism, a higher accumulation of triacylglycerols was not detected, but the cells did contain large lipid bodies, which suggests that small lipid bodies became fused into big lipid droplets. Vacuoles showed a similar behavior as the lipid bodies, and double labeling with Blue-CMAC and BODIPY indicates that vacuoles and lipid bodies were independent organelles. The results suggest that TORC1 has a role in cell morphology, lipid metabolism, and vacuolar physiology in U. maydis.
AB - The evolutionarily conserved serine/threonine kinase TOR recruits different subunits to assemble the Target of Rapamycin Complex 1 (TORC1), which is inhibited by rapamycin and regulates ribosome biogenesis, autophagy, and lipid metabolism by regulating the expression of lipogenic genes. In addition, TORC1 participates in the cell cycle, increasing the length of the G2 phase. In the present work, we investigated the effect of rapamycin on cell growth, cell morphology and neutral lipid metabolism in the phytopathogenic fungus Ustilago maydis. Inhibition of TORC1 by rapamycin induced the formation of septa that separate the nuclei that were formed after mitosis. Regarding neutral lipid metabolism, a higher accumulation of triacylglycerols was not detected, but the cells did contain large lipid bodies, which suggests that small lipid bodies became fused into big lipid droplets. Vacuoles showed a similar behavior as the lipid bodies, and double labeling with Blue-CMAC and BODIPY indicates that vacuoles and lipid bodies were independent organelles. The results suggest that TORC1 has a role in cell morphology, lipid metabolism, and vacuolar physiology in U. maydis.
KW - Lipid droplets
KW - Rapamycin
KW - TOR
KW - Ustilago maydis
KW - Vacuoles
UR - http://www.scopus.com/inward/record.url?scp=85079599048&partnerID=8YFLogxK
U2 - 10.1007/s00203-020-01833-y
DO - 10.1007/s00203-020-01833-y
M3 - Artículo
C2 - 32088730
AN - SCOPUS:85079599048
SN - 0302-8933
VL - 202
SP - 1211
EP - 1221
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 5
ER -