TY - JOUR
T1 - Promysalin, a salicylate-containing pseudomonas putida antibiotic, promotes surface colonization and selectively targets other pseudomonas
AU - Li, Wen
AU - Estrada-De Los Santos, Paulina
AU - Matthijs, Sandra
AU - Xie, Guan Lin
AU - Busson, Roger
AU - Cornelis, Pierre
AU - Rozenski, Jef
AU - De Mot, René
N1 - Funding Information:
This work was supported by Grant G.0303.04N from FWO-Vlaanderen (to P.C. and R.DM.), by a K.U.Leuven-Zhejiang University interuniversity SBA fellowship to W.L. (K.U.Leuven Research Council Fund), and by a junior postdoctoral fellowship F/04/026-F/05/32 to P.E-dlS. (K.U.Leuven Research Council Fund). The authors thank Sarah Denayer and Hassan Rokni-Zadeh for antagonism assays with P. aeruginosa and P. savastanoi , respectively, and Wesley Mattheus for advice on construction of unmarked deletion mutants. The assistance of Luc Baudemprez for recording NMR spectra and Xiaoping Song with chemical analyses is much appreciated. Guido Bloemberg kindly provided plasmid pMP6562.
PY - 2011/10/28
Y1 - 2011/10/28
N2 - Under control of the Gac regulatory system, Pseudomonas putida RW10S1 produces promysalin to promote its own swarming and biofilm formation, and to selectively inhibit many other pseudomonads, including the opportunistic pathogen Pseudomonas aeruginosa. This amphipathic antibiotic is composed of salicylic acid and 2,8-dihydroxymyristamide bridged by a unique 2-pyrroline-5-carboxyl moiety. In addition to enzymes for salicylic acid synthesis and activation, the biosynthetic gene cluster encodes divergent type II fatty acid biosynthesis components, unusual fatty acid-tailoring enzymes (two Rieske-type oxygenases and an amidotransferase), an enzyme resembling a proline-loading module of nonribosomal peptide synthetases, and the first prokaryotic member of the BAHD family of plant acyltransferases. Identification of biosynthetic intermediates enabled to propose a pathway for synthesis of this bacterial colonization factor.
AB - Under control of the Gac regulatory system, Pseudomonas putida RW10S1 produces promysalin to promote its own swarming and biofilm formation, and to selectively inhibit many other pseudomonads, including the opportunistic pathogen Pseudomonas aeruginosa. This amphipathic antibiotic is composed of salicylic acid and 2,8-dihydroxymyristamide bridged by a unique 2-pyrroline-5-carboxyl moiety. In addition to enzymes for salicylic acid synthesis and activation, the biosynthetic gene cluster encodes divergent type II fatty acid biosynthesis components, unusual fatty acid-tailoring enzymes (two Rieske-type oxygenases and an amidotransferase), an enzyme resembling a proline-loading module of nonribosomal peptide synthetases, and the first prokaryotic member of the BAHD family of plant acyltransferases. Identification of biosynthetic intermediates enabled to propose a pathway for synthesis of this bacterial colonization factor.
UR - http://www.scopus.com/inward/record.url?scp=80055082464&partnerID=8YFLogxK
U2 - 10.1016/j.chembiol.2011.08.006
DO - 10.1016/j.chembiol.2011.08.006
M3 - Artículo
SN - 1074-5521
VL - 18
SP - 1320
EP - 1330
JO - Chemistry and Biology
JF - Chemistry and Biology
IS - 10
ER -