TY - JOUR
T1 - Production of plant proteases in vivo and in vitro - A review
AU - González-Rábade, Nuria
AU - Badillo-Corona, Jesús Agustín
AU - Aranda-Barradas, Juan Silvestre
AU - Oliver-Salvador, María del Carmen
N1 - Funding Information:
Research in the laboratory of JABC and the postdoctoral visit of NGR are sponsored by CONACyT-Mexico ( CB-2008-01 ; 0104529 ) and Instituto Politécnico Nacional-SIP ( 20100711 and 20100711 ).
PY - 2011/11
Y1 - 2011/11
N2 - In the latest two decades, the interest received by plant proteases has increased significantly. Plant enzymes such as proteases are widely used in medicine and the food industry. Some proteases, like papain, bromelain and ficin are used in various processes such as brewing, meat softening, milk-clotting, cancer treatment, digestion and viral disorders. These enzymes can be obtained from their natural source or through in vitro cultures, in order to ensure a continuous source of plant enzymes. The focus of this review will be the production of plant proteases both in vivo and in vitro, with particular emphasis on the different types of commercially important plant proteases that have been isolated and characterized from naturally grown plants. In vitro approaches for the production of these proteases is also explored, focusing on the techniques that do not involve genetic transformation of the plants and the attempts that have been made in order to enhance the yield of the desired proteases.
AB - In the latest two decades, the interest received by plant proteases has increased significantly. Plant enzymes such as proteases are widely used in medicine and the food industry. Some proteases, like papain, bromelain and ficin are used in various processes such as brewing, meat softening, milk-clotting, cancer treatment, digestion and viral disorders. These enzymes can be obtained from their natural source or through in vitro cultures, in order to ensure a continuous source of plant enzymes. The focus of this review will be the production of plant proteases both in vivo and in vitro, with particular emphasis on the different types of commercially important plant proteases that have been isolated and characterized from naturally grown plants. In vitro approaches for the production of these proteases is also explored, focusing on the techniques that do not involve genetic transformation of the plants and the attempts that have been made in order to enhance the yield of the desired proteases.
KW - In vitro
KW - In vivo
KW - Plant proteases
UR - http://www.scopus.com/inward/record.url?scp=80053439470&partnerID=8YFLogxK
U2 - 10.1016/j.biotechadv.2011.08.017
DO - 10.1016/j.biotechadv.2011.08.017
M3 - Artículo de revisión
SN - 0734-9750
VL - 29
SP - 983
EP - 996
JO - Biotechnology Advances
JF - Biotechnology Advances
IS - 6
ER -