TY - JOUR
T1 - Production of hybrid strains among Pleutorus and Lentinula and evaluation of their mycelial growth kinetics on malt extract agar and wheat grain using the Gompertz and Hill models
AU - Cobos, Juan Diego Valenzuela
AU - Páramo, Enrique Durán
AU - Arce, Ramón Villanueva
AU - Aguilar, María Eugenia Garín
AU - Hernández, Abraham Sánchez
AU - Lara, Hermilo Leal
AU - Del Toro, Gustavo Valencia
N1 - Funding Information:
Financial support received for: IPN-SIP Project: 20170419, CONACYT Project: CB-2016-611914
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Three inter-generic hybrid strains were obtained by pairing compatible neohaplonts recovered by dedikaryotization of four parental strains, i.e. Pleurotus ostreatus (CC060), Pleurotus djamor (CC051) and Lentinula edodes (CC003 and CC004). Fifteen neohaplonts were recovered employing homogenization time periods for Pleurotus spp. since 60 to 90 s, and for Lentinula edodes strains time periods ranged from 5 to 20 s, and incubation at 28 °C in a peptone-glucose solution (PGS). The mycelial growth of the parental and hybrid strains was determined on malt extract agar (MEA) and wheat grain by calculating the parameters A, B and C of nonlinear regression models. For the parameter A, the mycelial growth on MEA showed values ranged from 1.08 to 1.28, and for the parameter B since 5.65 to 20.85. On the other hand, the A values by mycelium growth on wheat grain ranged from 1.57 to 16.13, for B since 28.36 to 86.53 and for C were in an interval from 11.89 to 44.24. Parental and hybrid strains presented instantaneous velocity values on MEA since 2.88 to 7.26 cm2•day-1, whereas on wheat grain were in an interval from 5.45 to 10.05 cm3•day-1. Furthemore, the μmax values on MEA and wheat grain were calculated by using the Gompertz model and the λ values on both medium were estimated by using the Hill model, the μmax values on MEA ranged from 0.26 to 1.69 day-1 and λ values in an interval from 0.41 to 2.74, whereas the μmax values on wheat grain ranged from 0.05 to 0.43 day-1 and λ since 1.82 to 28 h. Moreover, the estimated equations based on nonlinear models were used to calculate the μmax values on MEA and wheat grain of the strains and the λ values were obtained by using the μmax values of the proposed equations on the Hill model, the μmax values on MEA ranged from 0.44 to 1.27 day-1 andthe λ values between 0.57 to 1.72 h, while the μmax values on wheat grain ranged from 0.04 to 0.64 day-1 and λ values in an interval from 1.26 to 20.11 h. The results evidenced that the hybrid strains of PleurotusxLentinula presented highest rate of growth in comparison with the parental, encouraging the production of hybrid strain and their use in the industrial field.
AB - Three inter-generic hybrid strains were obtained by pairing compatible neohaplonts recovered by dedikaryotization of four parental strains, i.e. Pleurotus ostreatus (CC060), Pleurotus djamor (CC051) and Lentinula edodes (CC003 and CC004). Fifteen neohaplonts were recovered employing homogenization time periods for Pleurotus spp. since 60 to 90 s, and for Lentinula edodes strains time periods ranged from 5 to 20 s, and incubation at 28 °C in a peptone-glucose solution (PGS). The mycelial growth of the parental and hybrid strains was determined on malt extract agar (MEA) and wheat grain by calculating the parameters A, B and C of nonlinear regression models. For the parameter A, the mycelial growth on MEA showed values ranged from 1.08 to 1.28, and for the parameter B since 5.65 to 20.85. On the other hand, the A values by mycelium growth on wheat grain ranged from 1.57 to 16.13, for B since 28.36 to 86.53 and for C were in an interval from 11.89 to 44.24. Parental and hybrid strains presented instantaneous velocity values on MEA since 2.88 to 7.26 cm2•day-1, whereas on wheat grain were in an interval from 5.45 to 10.05 cm3•day-1. Furthemore, the μmax values on MEA and wheat grain were calculated by using the Gompertz model and the λ values on both medium were estimated by using the Hill model, the μmax values on MEA ranged from 0.26 to 1.69 day-1 and λ values in an interval from 0.41 to 2.74, whereas the μmax values on wheat grain ranged from 0.05 to 0.43 day-1 and λ since 1.82 to 28 h. Moreover, the estimated equations based on nonlinear models were used to calculate the μmax values on MEA and wheat grain of the strains and the λ values were obtained by using the μmax values of the proposed equations on the Hill model, the μmax values on MEA ranged from 0.44 to 1.27 day-1 andthe λ values between 0.57 to 1.72 h, while the μmax values on wheat grain ranged from 0.04 to 0.64 day-1 and λ values in an interval from 1.26 to 20.11 h. The results evidenced that the hybrid strains of PleurotusxLentinula presented highest rate of growth in comparison with the parental, encouraging the production of hybrid strain and their use in the industrial field.
KW - Dedikaryotization
KW - Mushroom
KW - Mycelium growth
KW - Neohaplonts
KW - Strains
UR - http://www.scopus.com/inward/record.url?scp=85040687646&partnerID=8YFLogxK
U2 - 10.9755/ejfa.2017.v29.i12.1563
DO - 10.9755/ejfa.2017.v29.i12.1563
M3 - Artículo
SN - 2079-052X
VL - 29
SP - 927
EP - 935
JO - Emirates Journal of Food and Agriculture
JF - Emirates Journal of Food and Agriculture
IS - 12
ER -