TY - JOUR
T1 - Portable device for magnetic stimulation
T2 - Assessment survival and proliferation in human lymphocytes
AU - Pérez, H.
AU - Cordova-Fraga, T.
AU - López-Briones, S.
AU - Martínez-Espinosa, J. C.
AU - Rosas, E. F.
AU - Espinoza, A.
AU - Villagómez-Castro, J. C.
AU - Sosa, M.
AU - Topsu, S.
AU - Bernal-Alvarado, J. J.
N1 - Funding Information:
The authors thank the Universidad de Guanajuato campus León, Transversal Project 2012, and DAIP Institutional Projects 2012 for the financial support.
PY - 2013/9
Y1 - 2013/9
N2 - A device's instrumentation for magnetic stimulation on human lymphocytes is presented. This is a new procedure to stimulate growing cells with ferrofluid in vortices of magnetic field. The stimulation of magnetic vortices was provided at five different frequencies, from 100 to 2500 Hz and intensities from 1.13 to 4.13 mT. To improve the stimulation effects, a paramagnetic ferrofluid was added on the cell culture medium. The results suggest that the frequency changes and the magnetic field variation produce an important increase in the number of proliferating cells as well as in the cellular viability. This new magnetic stimulation modality could trigger an intracellular mechanism to induce cell proliferation and cellular survival only on mitogen stimulated cells.
AB - A device's instrumentation for magnetic stimulation on human lymphocytes is presented. This is a new procedure to stimulate growing cells with ferrofluid in vortices of magnetic field. The stimulation of magnetic vortices was provided at five different frequencies, from 100 to 2500 Hz and intensities from 1.13 to 4.13 mT. To improve the stimulation effects, a paramagnetic ferrofluid was added on the cell culture medium. The results suggest that the frequency changes and the magnetic field variation produce an important increase in the number of proliferating cells as well as in the cellular viability. This new magnetic stimulation modality could trigger an intracellular mechanism to induce cell proliferation and cellular survival only on mitogen stimulated cells.
UR - http://www.scopus.com/inward/record.url?scp=84885170768&partnerID=8YFLogxK
U2 - 10.1063/1.4819796
DO - 10.1063/1.4819796
M3 - Artículo
C2 - 24089844
SN - 0034-6748
VL - 84
JO - Review of Scientific Instruments
JF - Review of Scientific Instruments
IS - 9
M1 - 094701
ER -