TY - JOUR
T1 - Piper auritum Kunth (Piperaceae) improves the sexual performance of sluggish male rats through enhancing ejaculation
AU - Estrada-Reyes, Rosa
AU - Dorantes-Barrón, Ana María
AU - Arrieta-Báez, Daniel
AU - Gómez-Patiño, Mayra Beatriz
AU - Bernal-Trujillo, Andrea
AU - Castro-García, Mario
AU - Carro-Juárez, Miguel
AU - Martínez-Mota, Lucía
N1 - Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - Ethnopharmacological relevance: Piper auritum Kunth is employed as an aphrodisiac in the traditional medicine, but corroborative evidence for such effect is scarce. Aim of the study: The pro-sexual effect of an aqueous extract of P. auritum and its possible mechanisms were analyzed in two paradigms of male sexual function. Material and methods: Effects of an aqueous extract of P. auritum (PA, single administration) were investigated in the fictive ejaculation, and copulatory behavior paradigms in sexually sluggish male rats. WAY 100635 (antagonist of 5-HT1A receptors), atosiban (antagonist of oxytocinergic receptors), L-NAME (inhibitor of the nitric oxide synthase) and baclofen (antagonist of GABAB receptors) were used as pre-treatments in order to investigate the role of different neurotransmitter systems in PA actions. Chemical profile of PA was determined by Gases Chromatography and Ultra Performance Chromatography-Electrospray Ionization-Masses Spectrometry (UPLC-ESI-MS). Results: In males with retarded ejaculation, PA stimulated ejaculatory behavior and recovered electromyographic activity of pelvic musculature participating in seminal emission and ejaculation. All pre-treatments blocked stimulating effects of PA on the fictive ejaculation; additionally WAY 100635 interfered with PA actions on ejaculatory behavior. Safrol, apigenin dimethylether, myristicin, vaccihein A, sakuranin and sakuranetin flavonoids, were main constituents of PA, with possible participation in its pro-sexual effects. Conclusions: Pro-sexual effects of P. auritum elicited at level of ejaculation were mediated by several neurotransmitter systems, among which serotonin and its 5-HT1A receptors play an important role. Present findings support P. auritum reputation as an aphrodisiac, with potential use in delayed ejaculation disorder.
AB - Ethnopharmacological relevance: Piper auritum Kunth is employed as an aphrodisiac in the traditional medicine, but corroborative evidence for such effect is scarce. Aim of the study: The pro-sexual effect of an aqueous extract of P. auritum and its possible mechanisms were analyzed in two paradigms of male sexual function. Material and methods: Effects of an aqueous extract of P. auritum (PA, single administration) were investigated in the fictive ejaculation, and copulatory behavior paradigms in sexually sluggish male rats. WAY 100635 (antagonist of 5-HT1A receptors), atosiban (antagonist of oxytocinergic receptors), L-NAME (inhibitor of the nitric oxide synthase) and baclofen (antagonist of GABAB receptors) were used as pre-treatments in order to investigate the role of different neurotransmitter systems in PA actions. Chemical profile of PA was determined by Gases Chromatography and Ultra Performance Chromatography-Electrospray Ionization-Masses Spectrometry (UPLC-ESI-MS). Results: In males with retarded ejaculation, PA stimulated ejaculatory behavior and recovered electromyographic activity of pelvic musculature participating in seminal emission and ejaculation. All pre-treatments blocked stimulating effects of PA on the fictive ejaculation; additionally WAY 100635 interfered with PA actions on ejaculatory behavior. Safrol, apigenin dimethylether, myristicin, vaccihein A, sakuranin and sakuranetin flavonoids, were main constituents of PA, with possible participation in its pro-sexual effects. Conclusions: Pro-sexual effects of P. auritum elicited at level of ejaculation were mediated by several neurotransmitter systems, among which serotonin and its 5-HT1A receptors play an important role. Present findings support P. auritum reputation as an aphrodisiac, with potential use in delayed ejaculation disorder.
KW - Acuyo
KW - Aphrodisiac
KW - Atosiban (PubChem CID: 5311010)
KW - Baclofen (PubChem CID: 2284)
KW - Ejaculatory function
KW - Estradiol benzoate (Pubchem CID: 222757)
KW - Hierba santa
KW - Male sexual behavior
KW - N(ω)-nitro-L-arginine methyl ester (PubChem CID: 39836)
KW - PTF, polytetrafluoroethylene (PubChem CID: 8301)
KW - Progesterone (PubChem CID: 5994)
KW - Serotonergic receptors
KW - Sildenafil citrate (PubChem CID: 62853)
KW - Urethane (PubChem CID: 5641)
KW - WAY 100635 (PubChem CID: 5684)
UR - http://www.scopus.com/inward/record.url?scp=85057976024&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2018.10.030
DO - 10.1016/j.jep.2018.10.030
M3 - Artículo
C2 - 30545804
SN - 0378-8741
VL - 231
SP - 453
EP - 463
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
ER -