TY - JOUR
T1 - Peripheral blood mobilization of different lymphocyte and dendritic cell subsets with the use of intermediate doses of G-CSF in patients with non-Hodgkin's lymphoma and multiple myeloma
AU - Vela-Ojeda, J.
AU - García-Ruiz Esparza, M. A.
AU - Reyes-Maldonado, E.
AU - Jiménez-Zamudio, L.
AU - García-Latorre, E.
AU - Moreno-Lafont, M.
AU - Estrada-García, I.
AU - Mayani, H.
AU - Montiel-Cervantes, L.
AU - Tripp-Villanueva, F.
AU - Ayala-Sánchez, M.
AU - García-León, L. D.
AU - Borbolla-Escoboza, J. R.
N1 - Funding Information:
Acknowledgement This work was partially supported by grant No. 2004-123 from the FOFOI (Fondo de Fomento a la Investigación, Dirección de Prestaciones Médicas, Coordinación de Investigación en Salud, Instituto Mexicano del Seguro Social, México). The experiments performed in the present paper complied with the current laws of Mexico and were approved by the Ethical Committee in La Raza Medical Center.
PY - 2006/5
Y1 - 2006/5
N2 - Between June 2003 and November 2004, we collected mobilized peripheral blood units from 29 patients with non-Hodgkin's lymphoma and multiple myeloma for autologous peripheral blood stem cell transplantation. They received granulocyte colony-stimulating factor (G-CSF) (16 μg/kg/day) for a total of 5 days. Immediately before and 3 h after the fourth and fifth dose of G-CSF, we performed flow cytometry analysis to quantify: T cells (CD3+CD4+, CD3+CD8+), B cells (CD19+), NK cells (CD3-CD16+CD56+), NKT cells (CD3+CD16+CD56+), type 1 dendritic cells (DC1) (lin-HLA-DR+CD11c+), type 2 dendritic cells (DC2) (lin-HLA-DR+CD123+), regulatory T cells (Tregs) (CD4+CD25+), and activated T cells (CD3+HLA-DR+). All cell subsets were mobilized after G-CSF treatment with the exception of B, NK, and NKT lymphocytes. The median number of Treg cells before and after G-CSF was statistically different (29±14.9×106/l vs 70.1±46.1×106/l, P<0.02). DCs were mobilized significantly with a 5.9-fold increase in DC2 (15.1±30.3×106/l vs 89.8±81.0×106/l, P<0.02) and a 2.6-fold increase for DC1 (41±42.5×106/l vs 109.5±58.0×106/l, P<0.04). Patients received a mean of 3.1±1.2×107/kg NK cells, 1.3±0.9×107/kg NKT cells, 0.41±0.29×107/kg DC1, 0.2±0.22×107/kg DC2, and 1.8±1.9×107/kg Tregs. In conclusion, intermediate doses of G-CSF induce mobilization of different lymphocyte subsets, with the exception of B, NK, and NKT cells. The mobilization of certain suppressive populations (DC2 and Treg) could be in theory deleterious, at least in patients with cancer.
AB - Between June 2003 and November 2004, we collected mobilized peripheral blood units from 29 patients with non-Hodgkin's lymphoma and multiple myeloma for autologous peripheral blood stem cell transplantation. They received granulocyte colony-stimulating factor (G-CSF) (16 μg/kg/day) for a total of 5 days. Immediately before and 3 h after the fourth and fifth dose of G-CSF, we performed flow cytometry analysis to quantify: T cells (CD3+CD4+, CD3+CD8+), B cells (CD19+), NK cells (CD3-CD16+CD56+), NKT cells (CD3+CD16+CD56+), type 1 dendritic cells (DC1) (lin-HLA-DR+CD11c+), type 2 dendritic cells (DC2) (lin-HLA-DR+CD123+), regulatory T cells (Tregs) (CD4+CD25+), and activated T cells (CD3+HLA-DR+). All cell subsets were mobilized after G-CSF treatment with the exception of B, NK, and NKT lymphocytes. The median number of Treg cells before and after G-CSF was statistically different (29±14.9×106/l vs 70.1±46.1×106/l, P<0.02). DCs were mobilized significantly with a 5.9-fold increase in DC2 (15.1±30.3×106/l vs 89.8±81.0×106/l, P<0.02) and a 2.6-fold increase for DC1 (41±42.5×106/l vs 109.5±58.0×106/l, P<0.04). Patients received a mean of 3.1±1.2×107/kg NK cells, 1.3±0.9×107/kg NKT cells, 0.41±0.29×107/kg DC1, 0.2±0.22×107/kg DC2, and 1.8±1.9×107/kg Tregs. In conclusion, intermediate doses of G-CSF induce mobilization of different lymphocyte subsets, with the exception of B, NK, and NKT cells. The mobilization of certain suppressive populations (DC2 and Treg) could be in theory deleterious, at least in patients with cancer.
KW - Dendritic cells
KW - G-CSF
KW - Mobilization
KW - Regulatory T cells
UR - http://www.scopus.com/inward/record.url?scp=33646713892&partnerID=8YFLogxK
U2 - 10.1007/s00277-006-0090-8
DO - 10.1007/s00277-006-0090-8
M3 - Artículo
C2 - 16525786
SN - 0939-5555
VL - 85
SP - 308
EP - 314
JO - Annals of Hematology
JF - Annals of Hematology
IS - 5
ER -