TY - JOUR
T1 - Ozone exposure induces iNOS expression and tyrosine nitration in rat aorta
AU - Sánchez-González, Dolores J.
AU - Moro, María A.
AU - Castillo-Henkel, Carlos
AU - Herrera-González, Norma
AU - Hernández-Pando, Rogelio
AU - Larios-Medina, Francisco J.
AU - Cobilt, Rafael
AU - Blanco, José A.
AU - Pedraza-Chaverrí, José
AU - Villanueva, Cleva
N1 - Funding Information:
This work was supported by Instituto Politécnico Nacional (CGPI 20010692, CGPI 20021236, CGPI 20030233) and Universidad del Ejército y Fuerza Aérea. Animals were provided by Dr. Selva Rivas Arancibia, Universidad Nacional Autónoma de México.
PY - 2004/5
Y1 - 2004/5
N2 - The aim was to study whether ozone affects vascular endothelium by causing inducible nitric oxide synthase (iNOS) expression and tyrosine nitration. We also studied biomarkers of endothelial function. Male Wistar rats were exposed to ozone (0.25 ppm, 4 h/day) or filtered air (control, ozone <0.05 ppm). After ozone exposure, blood samples were taken to measure 6-keto prostaglandin F1α (6-keto PGF1α), dehydro-thromboxane B2 (DH-TxB 2), endothelin-1 and NO2-/NO3 - (NOx-). iNOS and nitrotyrosine were detected in aorta by immunohistochemistry. Nitrotyrosine was also detected by immunoelectromicroscopy. Control aortae failed to show either iNOS or nitrotyrosine. Time-dependent positive iNOS and nitrotyrosine cells were observed in exposed animals. Except for NOx-, endothelial markers decreased after 14 days of ozone exposure (P<0.05). After 28 days of ozone, 6-keto PGF1α remained low (P<0.05) while DH-TxB2 increased (P<0.05). It is concluded that ozone causes endothelial dysfunction manifested early with peroxynitrite formation and lately with changes in endothelial markers.
AB - The aim was to study whether ozone affects vascular endothelium by causing inducible nitric oxide synthase (iNOS) expression and tyrosine nitration. We also studied biomarkers of endothelial function. Male Wistar rats were exposed to ozone (0.25 ppm, 4 h/day) or filtered air (control, ozone <0.05 ppm). After ozone exposure, blood samples were taken to measure 6-keto prostaglandin F1α (6-keto PGF1α), dehydro-thromboxane B2 (DH-TxB 2), endothelin-1 and NO2-/NO3 - (NOx-). iNOS and nitrotyrosine were detected in aorta by immunohistochemistry. Nitrotyrosine was also detected by immunoelectromicroscopy. Control aortae failed to show either iNOS or nitrotyrosine. Time-dependent positive iNOS and nitrotyrosine cells were observed in exposed animals. Except for NOx-, endothelial markers decreased after 14 days of ozone exposure (P<0.05). After 28 days of ozone, 6-keto PGF1α remained low (P<0.05) while DH-TxB2 increased (P<0.05). It is concluded that ozone causes endothelial dysfunction manifested early with peroxynitrite formation and lately with changes in endothelial markers.
KW - Endothelial dysfunction
KW - Oxidative stress
KW - Ozone
KW - Peroxynitrite
UR - http://www.scopus.com/inward/record.url?scp=2342512229&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2004.01.002
DO - 10.1016/j.etap.2004.01.002
M3 - Artículo
SN - 1382-6689
VL - 17
SP - 1
EP - 7
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
IS - 1
ER -