Resumen
Isolated from oil-contaminated soil, Pseudomonas aeruginosa strain MGP-1 was identified by 16S rDNA sequence analysis and phenotypic characterisation. This strain was highly competent in medium and in soils, grew and survived well under adverse conditions. MGP-1 did not produce the pathogenic factor pyocyanin and used paraffin (C11-40) as sole carbon source. It degraded eicosane (C20) more efficiently than other n-alkanes, removing 60% within 16 days. An alkB gene and intermediate metabolites of mono-alcohol and lipid acid corresponding to the oxidised paraffin were detected in the cell-free extracts of MGP-1, indicating that it possessed a mono-terminal oxidation. This is the first report to demonstrate that a Pseudomonas strain is able to degrade paraffin with more than 18 carbon atoms by mono-terminal oxidation. Considering all the results, MGP-1 could be designed as a soil-born, highly competent, long chain n-alkane degrading bacterium with low or perhaps without pathogenicity.
Idioma original | Inglés |
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Páginas (desde-hasta) | 321-328 |
Número de páginas | 8 |
Publicación | Annals of Microbiology |
Volumen | 57 |
N.º | 3 |
DOI | |
Estado | Publicada - 2007 |