On the use of n-octyl gallate and salicylhydroxamic acid to study the alternative oxidase role

Lucero Romero-Aguilar, Christian Cárdenas-Monroy, Verónica Garrido-Bazán, Jesus Aguirre, Guadalupe Guerra-Sánchez, Juan Pablo Pardo

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

Resumen

The alternative oxidase (AOX) catalyzes the transfer of electrons from ubiquinol to oxygen without the translocation of protons across the inner mitochondrial membrane. This enzyme has been proposed to participate in the regulation of cell growth, sporulation, yeast-mycelium transition, resistance to reactive oxygen species, infection, and production of secondary metabolites. Two approaches have been used to evaluate AOX function: incubation of cells for long periods of time with AOX inhibitors or deletion of AOX gene. However, AOX inhibitors might have different targets. To test non-specific effects of n-octyl gallate (nOg) and salicylhydroxamic acid (SHAM) on fungal physiology we measured the growth and respiratory capacity of two fungal strains lacking (Ustilago maydis-Δaox and Saccharomyces cerevisiae) and three species containing the AOX gene (U. maydis WT, Debaryomyces hansenii, and Aspergillus nidulans). For U. maydis, a strong inhibition of growth and respiratory capacity by SHAM was observed, regardless of the presence of AOX. Similarly, A. nidulans mycelial growth was inhibited by low concentrations of nOg independently of AOX expression. In contrast, these inhibitors had no effect or had a minor effect on S. cerevisiae and D. hansenii growth. These results show that nOg and SHAM have AOX independent effects which vary in different microorganisms, indicating that studies based on long-term incubation of cells with these inhibitors should be considered as inconclusive.

Idioma originalInglés
Número de artículo108603
PublicaciónArchives of Biochemistry and Biophysics
Volumen694
DOI
EstadoPublicada - 15 nov. 2020

Huella

Profundice en los temas de investigación de 'On the use of n-octyl gallate and salicylhydroxamic acid to study the alternative oxidase role'. En conjunto forman una huella única.

Citar esto