TY - JOUR
T1 - Obtaining of astaxanthin from crab exosqueletons and shrimp head shells
AU - Montoya, Jorge Montiel
AU - Mata, Salvador Velazco
AU - Acosta, José Luis
AU - Cabrera, Braulio Edgar Herrera
AU - Valdez, Luis Germán López
AU - Reyes, César
AU - Cureño, Hebert Jair Barrales
N1 - Publisher Copyright:
© 2021 by the authors.
PY - 2021
Y1 - 2021
N2 - Fresh wastes of Mexican headshell shrimp (Litopenaeus vannamei), blue crab (Callinectes bellicosus), and Mexican brown crab (Callinectes sapidus) exoskeletons were autoclaved, dried, and grounded at a particle size of 150 µm. Macerated samples were diluted to ethyl acetate, acetone, cyclohexane, isopropyl alcohol, hexane, heptane, and a combination of hexane-acetone-ethanol-toluene solvents. High-Performance Liquid Chromatography analysis determined the astaxanthin amount in every organic solvent extract. Acetone was the most efficient solvent: 114 µg/g (L. vannamei), 39 µg/g (C. bellicosus), and 44 µg/g (C. sapidus); the mixture of hexane-acetone-ethanol-toluene resulted a idoneus solvent when was used on crab exoskeletons: 39 µg/g (C. bellicosus) and 51 µg/g (C. sapidus). The astaxanthin characterization was performed without saponification, in L. vannamei chromatograms, the amount of trans astaxanthin was 6.23 µg/g (5.47 % of total area), in C. bellicosus was 26.13 µg/g (67 % of total area) and in C. sapidus was 28.42 µg/g (64.6 % of total area).
AB - Fresh wastes of Mexican headshell shrimp (Litopenaeus vannamei), blue crab (Callinectes bellicosus), and Mexican brown crab (Callinectes sapidus) exoskeletons were autoclaved, dried, and grounded at a particle size of 150 µm. Macerated samples were diluted to ethyl acetate, acetone, cyclohexane, isopropyl alcohol, hexane, heptane, and a combination of hexane-acetone-ethanol-toluene solvents. High-Performance Liquid Chromatography analysis determined the astaxanthin amount in every organic solvent extract. Acetone was the most efficient solvent: 114 µg/g (L. vannamei), 39 µg/g (C. bellicosus), and 44 µg/g (C. sapidus); the mixture of hexane-acetone-ethanol-toluene resulted a idoneus solvent when was used on crab exoskeletons: 39 µg/g (C. bellicosus) and 51 µg/g (C. sapidus). The astaxanthin characterization was performed without saponification, in L. vannamei chromatograms, the amount of trans astaxanthin was 6.23 µg/g (5.47 % of total area), in C. bellicosus was 26.13 µg/g (67 % of total area) and in C. sapidus was 28.42 µg/g (64.6 % of total area).
KW - Acetone extract
KW - HPLC analysis
KW - Saponification
UR - http://www.scopus.com/inward/record.url?scp=85101068084&partnerID=8YFLogxK
U2 - 10.33263/BRIAC115.1351613523
DO - 10.33263/BRIAC115.1351613523
M3 - Artículo
AN - SCOPUS:85101068084
SN - 2069-5837
VL - 11
SP - 13516
EP - 13523
JO - Biointerface Research in Applied Chemistry
JF - Biointerface Research in Applied Chemistry
IS - 5
ER -