TY - JOUR
T1 - Nordihydroguaiaretic acid is a potent in vitro scavenger of peroxynitrite, singlet oxygen, hydroxyl radical, superoxide anion and hypochlorous acid and prevents in vivo ozone-induced tyrosine nitration in lungs
AU - Floriano-Sánchez, Esaú
AU - Villanueva, Cleva
AU - Medina-Campos, Omar Noel
AU - Rocha, Diana
AU - Sanchez-González, Dolores Javier
AU - Cardenas-Rodríguez, Noemí
AU - Pedraza-Chaverrí, José
N1 - Funding Information:
This work was supported by CONACYT (Grant No. 40009-M) and CGPI (Grant 20040088). EFS received a scholarship No 181778 from CONACYT and No. 2005-050 from PIFI. DJSG received a scholarship No 172018 from CONACYT. Axiovert 200 M confocal microscope was donated by Funda-cion Gonzalo Rio Arronte IAP Mexico.
PY - 2006/5
Y1 - 2006/5
N2 - The antioxidant nordihydroguaiaretic acid (NDGA) has recently become well known as a putative anticancer drug. In this paper, it was evaluated the in vitro peroxynitrite (ONOO-), singlet oxygen (1O2), hydroxyl radical (OH•), hydrogen peroxide (H2O2), superoxide anion (O2•- and hypochlorous acid (HOCl) scavenging capacity of NDGA. It was found that NDGA scavenges: (a) ONOO- (IC50 = 4 ± 0.94 μM) as efficiently as uric acid; (b) 1O2 (IC50 = 151 ± 20 μM) more efficiently than dimethyl thiourea, lipoic acid, N-acetyl-cysteine and glutathione; (c) OH• (IC50 = 0.15 ± 0.02 μM) more efficiently than dimethyl thiourea, uric acid, trolox, dimethyl sulfoxide and mannitol, (d) O2•- (IC50 = 15 ± 1 μM) more efficiently than N-acetyl-cysteine, glutathione, tempol and deferoxamine and (e) HOCl (IC50 = 622 ± 42 μ M) as efficiently as lipoic acid and N-acetyl-cysteine. NDGA was unable to scavenge H2 O2. In an in vivo study in rats, NDGA was able to prevent ozone-induced tyrosine nitration in lungs. It is concluded that NDGA is a potent in vitro scavenger of ONOO-, 1O2, OH•, O2•- and HOCl and is able to prevent lung tyrosine nitration in vivo.
AB - The antioxidant nordihydroguaiaretic acid (NDGA) has recently become well known as a putative anticancer drug. In this paper, it was evaluated the in vitro peroxynitrite (ONOO-), singlet oxygen (1O2), hydroxyl radical (OH•), hydrogen peroxide (H2O2), superoxide anion (O2•- and hypochlorous acid (HOCl) scavenging capacity of NDGA. It was found that NDGA scavenges: (a) ONOO- (IC50 = 4 ± 0.94 μM) as efficiently as uric acid; (b) 1O2 (IC50 = 151 ± 20 μM) more efficiently than dimethyl thiourea, lipoic acid, N-acetyl-cysteine and glutathione; (c) OH• (IC50 = 0.15 ± 0.02 μM) more efficiently than dimethyl thiourea, uric acid, trolox, dimethyl sulfoxide and mannitol, (d) O2•- (IC50 = 15 ± 1 μM) more efficiently than N-acetyl-cysteine, glutathione, tempol and deferoxamine and (e) HOCl (IC50 = 622 ± 42 μ M) as efficiently as lipoic acid and N-acetyl-cysteine. NDGA was unable to scavenge H2 O2. In an in vivo study in rats, NDGA was able to prevent ozone-induced tyrosine nitration in lungs. It is concluded that NDGA is a potent in vitro scavenger of ONOO-, 1O2, OH•, O2•- and HOCl and is able to prevent lung tyrosine nitration in vivo.
KW - Hydroxyl radical
KW - Nordihydroguaiaretic acid
KW - Peroxynitrite
KW - Scavenger
KW - Singlet oxygen
KW - Superoxide anion
UR - http://www.scopus.com/inward/record.url?scp=33645105351&partnerID=8YFLogxK
U2 - 10.1080/10715760500419365
DO - 10.1080/10715760500419365
M3 - Artículo
C2 - 16551579
SN - 1071-5762
VL - 40
SP - 523
EP - 533
JO - Free Radical Research
JF - Free Radical Research
IS - 5
ER -