TY - JOUR
T1 - Nordihydroguaiaretic acid attenuates potassium dichromate-induced oxidative stress and nephrotoxicity
AU - Yam-Canul, Paola
AU - Chirino, Yolanda I.
AU - Sánchez-González, Dolores Javier
AU - Martínez-Martínez, Claudia María
AU - Cruz, Cristino
AU - Villanueva, Cleva
AU - Pedraza-Chaverri, José
N1 - Funding Information:
This work was supported by CONACYT (Grant No. 48812). Axiovert 200 M confocal microscope was donated by Fundacion Gonzalo Rio Arronte IAP México.
PY - 2008/3
Y1 - 2008/3
N2 - Larrea tridentata also known as Creosote bush, Larrea, chaparral, greasewood or gobernadora has been used in the folk medicine for the treatment of several illnesses. The primary product that is present at high concentrations in the leaves from this plant is nordihydroguaiaretic acid (NDGA) which is a powerful antioxidant. On the other hand, potassium dichromate (K2Cr2O7)-induced nephrotoxicity is associated with oxidative stress. The aim of this work was to study the effect of NDGA on K2Cr2O7-induced nephrotoxicity and oxidative stress. Nephrotoxicity was induced by a single injection of K2Cr2O7 (15 mg/Kg). A group of K2Cr2O7-treated rats was administered NDGA by mini osmotic pumps (17 mg/Kg/day). The results show that NDGA was able to ameliorate the structural and functional renal damage evaluated by histopathological analysis and by measuring proteinuria, urinary excretion of N-acetyl-β-d-glucosaminidase, serum creatinine, and serum glutathione peroxidase activity. In addition, immunostaining of 4-hydroxy-2-nonenal and 3-nitrotyrosine, markers of oxidative and nitrosative stress, respectively, was ameliorated by the NDGA treatment. These data strongly suggest that the antioxidant properties of NDGA are involved in its renoprotective effect in K2Cr2O7-treated rats.
AB - Larrea tridentata also known as Creosote bush, Larrea, chaparral, greasewood or gobernadora has been used in the folk medicine for the treatment of several illnesses. The primary product that is present at high concentrations in the leaves from this plant is nordihydroguaiaretic acid (NDGA) which is a powerful antioxidant. On the other hand, potassium dichromate (K2Cr2O7)-induced nephrotoxicity is associated with oxidative stress. The aim of this work was to study the effect of NDGA on K2Cr2O7-induced nephrotoxicity and oxidative stress. Nephrotoxicity was induced by a single injection of K2Cr2O7 (15 mg/Kg). A group of K2Cr2O7-treated rats was administered NDGA by mini osmotic pumps (17 mg/Kg/day). The results show that NDGA was able to ameliorate the structural and functional renal damage evaluated by histopathological analysis and by measuring proteinuria, urinary excretion of N-acetyl-β-d-glucosaminidase, serum creatinine, and serum glutathione peroxidase activity. In addition, immunostaining of 4-hydroxy-2-nonenal and 3-nitrotyrosine, markers of oxidative and nitrosative stress, respectively, was ameliorated by the NDGA treatment. These data strongly suggest that the antioxidant properties of NDGA are involved in its renoprotective effect in K2Cr2O7-treated rats.
KW - Antioxidant
KW - Larrea tridentata
KW - Nephrotoxicity
KW - Oxidative stress
KW - Potassium dichromate
UR - http://www.scopus.com/inward/record.url?scp=38949125500&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2007.11.003
DO - 10.1016/j.fct.2007.11.003
M3 - Artículo
C2 - 18155343
SN - 0278-6915
VL - 46
SP - 1089
EP - 1096
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
IS - 3
ER -