TY - JOUR
T1 - Multiparameter flow cytometry analysis of leukocyte markers for diagnosis in preterm neonatal sepsis
AU - Vázquez Rodríguez, Stephania
AU - Arriaga Pizano, Lourdes A.
AU - Laresgoiti Servitje, Estibalitz
AU - Mancilla Ramirez, Javier
AU - Peralta Méndez, Omar L.
AU - Villalobos Alcazar, Gisela
AU - Granados Cepeda, Martha L.
AU - Hernandez Pelaez, Maria Graciela
AU - Cordero Gonzalez, Guadalupe
AU - Arizmendi Villanueva, Roberto
AU - Cruz Ramírez, José Luis
AU - Isibasi, Armando
AU - Lopez Macias, Constantino
AU - Flores Romo, Leopoldo
AU - Jimenez Zamudio, Luis A.
AU - Cérbulo-Vázquez, Arturo
N1 - Publisher Copyright:
© 2019 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2021
Y1 - 2021
N2 - Purpose: Neonatal sepsis is an important public health concern worldwide due to its immediate lethality and long-term morbidity rates, Clinical evaluation and laboratory analyses are indispensable for diagnosis of neonatal sepsis. However, assessing multiple biomarkers in neonates is difficult due to limited blood availability. The aim is to investigate if the neonatal sepsis in preterm could be identified by multiparameter analysis with flow cytometry. Materials and Methods: The expression of activation-related molecules was evaluated by flow cytometry in newborn with or without risk factors for sepsis. Results: Our analysis revealed that several markers could be useful for sepsis diagnosis, such as CD45RA, CD45RO, or CD71 on T cells; HLA-DR on NKT or classic monocytes, and TREM-1 on non-classic monocytes or neutrophils. However, ROC analysis shows that the expression of CD45RO on T lymphocytes is the only useful biomarker for diagnosis of neonatal late-onset sepsis. Also, decision tree analyses showed that CD45RO plus CD27 could help differentiate the preterm septic neonates from those with risk factors. Conclusions: Our study shows a complementary and practical strategy for biomarker assessment in neonatal sepsis.
AB - Purpose: Neonatal sepsis is an important public health concern worldwide due to its immediate lethality and long-term morbidity rates, Clinical evaluation and laboratory analyses are indispensable for diagnosis of neonatal sepsis. However, assessing multiple biomarkers in neonates is difficult due to limited blood availability. The aim is to investigate if the neonatal sepsis in preterm could be identified by multiparameter analysis with flow cytometry. Materials and Methods: The expression of activation-related molecules was evaluated by flow cytometry in newborn with or without risk factors for sepsis. Results: Our analysis revealed that several markers could be useful for sepsis diagnosis, such as CD45RA, CD45RO, or CD71 on T cells; HLA-DR on NKT or classic monocytes, and TREM-1 on non-classic monocytes or neutrophils. However, ROC analysis shows that the expression of CD45RO on T lymphocytes is the only useful biomarker for diagnosis of neonatal late-onset sepsis. Also, decision tree analyses showed that CD45RO plus CD27 could help differentiate the preterm septic neonates from those with risk factors. Conclusions: Our study shows a complementary and practical strategy for biomarker assessment in neonatal sepsis.
KW - Diagnosis
KW - flow cytometry
KW - markers
KW - neonate
KW - sepsis
UR - http://www.scopus.com/inward/record.url?scp=85073920156&partnerID=8YFLogxK
U2 - 10.1080/14767058.2019.1666100
DO - 10.1080/14767058.2019.1666100
M3 - Artículo
C2 - 31537145
AN - SCOPUS:85073920156
SN - 1476-7058
VL - 34
SP - 2323
EP - 2333
JO - Journal of Maternal-Fetal and Neonatal Medicine
JF - Journal of Maternal-Fetal and Neonatal Medicine
IS - 14
ER -