TY - JOUR
T1 - Multidrug resistance in the protozoan parasite Entamoeba histolytica
AU - Orozco, E.
AU - López, C.
AU - Gómez, C.
AU - Pérez, D. G.
AU - Marchat, L.
AU - Bañuelos, C.
AU - Delgadillo, D. M.
N1 - Funding Information:
The authors thank Alfredo Padilla-Barberi for his valuable help with the artwork. This paper was supported by CONACYT. EO is a Howard Hughes Medical Institute International Fellow.
PY - 2002/12
Y1 - 2002/12
N2 - In this review we discuss the mechanisms and molecules involved in the multidrug resistance (MDR) of the protozoan parasite Entamoeba histolytica. Drug resistant mutants exhibited the main characteristics presented by the MDR mammalian cells. They showed cross-resistance to several unrelated drugs that is reverted by calcium channel blockers. MDR phenotype in E. histolytica is regulated at a transcriptional level by the EhPgp1 gene, which is constitutively expressed and by the EhPgp5 gene, whose expression is induced in the presence of the drug. Transcription factors participate in the expression regulation of these genes. After over transcription, the EhPgp genes are amplified, cooperating to produce the MDR phenotype. Post-transcriptional mechanisms such as mRNA stability seem to be involved in this phenomenon. As for other mdr gene products, the EhPGP5 protein functions as a chloride current inductor or as a regulator of cellular regulatory volume decrease.
AB - In this review we discuss the mechanisms and molecules involved in the multidrug resistance (MDR) of the protozoan parasite Entamoeba histolytica. Drug resistant mutants exhibited the main characteristics presented by the MDR mammalian cells. They showed cross-resistance to several unrelated drugs that is reverted by calcium channel blockers. MDR phenotype in E. histolytica is regulated at a transcriptional level by the EhPgp1 gene, which is constitutively expressed and by the EhPgp5 gene, whose expression is induced in the presence of the drug. Transcription factors participate in the expression regulation of these genes. After over transcription, the EhPgp genes are amplified, cooperating to produce the MDR phenotype. Post-transcriptional mechanisms such as mRNA stability seem to be involved in this phenomenon. As for other mdr gene products, the EhPGP5 protein functions as a chloride current inductor or as a regulator of cellular regulatory volume decrease.
KW - Entamoeba histolytica
KW - Multidrug resistance
KW - Protozoan parasite
UR - http://www.scopus.com/inward/record.url?scp=0036888209&partnerID=8YFLogxK
U2 - 10.1016/S1383-5769(02)00041-7
DO - 10.1016/S1383-5769(02)00041-7
M3 - Artículo
SN - 1383-5769
VL - 51
SP - 353
EP - 359
JO - Parasitology International
JF - Parasitology International
IS - 4
ER -